中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (07): 849-854.DOI: 10.3724/SP.J.1095.2010.90604

• 研究论文 • 上一篇    下一篇

DNA对Ru(bpy)2(dppx)2+-SDS体系共振光散射的猝灭作用及其应用

黄剑平1*,梅平1,何治柯2   

  1. (1.长江大学化学与环境工程学院 荆州 434023; 2.武汉大学化学与分子科学学院 武汉)
  • 收稿日期:2009-09-08 修回日期:2009-11-21 出版日期:2010-07-10 发布日期:2010-07-10
  • 通讯作者: 黄剑平，男，硕士，副教授; E-mail:jianping1018@163.com; 研究方向：分子光谱分析
  • 基金资助:
    湖北省自然科学基金资助项目（2005ABA308）

Quenching Effect of Resonance Light Scattering of Ru(bpy)2(dppx)2+-SDS System by DNA and Its Application

HUANG Jian-Ping1*, MEI Ping1, HE Zhi-Ke2   

  1. (1.College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023;
    2.College of Chemistry and Molecular Sciences,Wuhan University,Wuhan)
  • Received:2009-09-08 Revised:2009-11-21 Published:2010-07-10 Online:2010-07-10

PDF

1180

被引次数

10

摘要：

研究了Ru(bpy)2(dppx)2+-SDS-DNA（bpy=2，2′-联吡啶，dppx=7,8-二甲基-吡啶并[3,2-a:2′,3′-c]吩嗪）体系的共振光散射光谱。结果表明，在阴离子表面活性剂十二烷基硫酸钠(SDS)预胶束聚集体存在下，Ru(bpy)2(dppx)2+-SDS体系具有很强的共振光散射，DNA的加入使其共振散射光猝灭。探讨了反应机理。基于DNA对Ru(bpy)2(dppx)2+-SDS体系共振光散射的猝灭作用，建立了共振光散射法测定DNA的新方法。在最佳实验条件下，体系在393 nm处的共振光散射猝灭程度与DNA的浓度呈线性关系，线性范围为0.01~1.2 μg/mL，检出限为1.5 μg/L。

关键词: Ru(bpy)2(dppx)2+, SDS, DNA, 共振光散射, 猝灭

Abstract:

The resonance light scattering（RLS） spectra of Ru(bpy)2(dppx)2+-SDS-DNA(bpy=2,2'-bipyridine; dppx=7,8-dimethyl-dipyrido[3,2-a:2',3'-c]phenazine) system was studied. The results show that the solution of

Ru(bpy)2(dppx)2+-SDS exhibits a high RLS signal in the presence of the premicellar aggregation of an anionic surfactant, sodium dodecyl sulphate(SDS), and adding DNA in the solution of Ru(bpy)2(dppx)2+-SDS results in the quenching of the RLS. The mechanism of the reaction was explored. Based on the quenching effect of DNA on the RLS of Ru(bpy)2(dppx)2+-SDS system, a novel RLS method for the determination of DNA was proposed. Under optimal conditions, the quenched intensity of RLS at 393 nm is proportional to the concentration of DNA. The linear range of the determination is 0.01~1.2 mg/L. The detection limit is 1.5 μg/L. This method was successful applied to the determination of DNA in synthetic and real samples.

Key words: Ru(bpy)2(dppx)2+, SDS, DNA, resonance light scattering, quench

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