应用化学 ›› 2023, Vol. 40 ›› Issue (2): 252-260.DOI: 10.19894/j.issn.1000-0518.220164

• 研究论文 • 上一篇    下一篇

基于抗菌性壳聚糖/羧甲基纤维素复合药物涂层的聚氨酯敷料

陈玉竹, 刘思思, 张蒙蒙, 林祥德(), 曾冬冬()   

  1. 上海健康医学院医疗器械学院,上海 201318
  • 收稿日期:2022-05-01 接受日期:2022-08-30 出版日期:2023-02-01 发布日期:2023-02-27
  • 通讯作者: 林祥德,曾冬冬
  • 基金资助:
    国家自然科学基金(22008151);上海市自然科学基金(19ZR1474300);上海健康医学院高水平地方高校建设项目

Polyurethane Dressing Based on Antibacterial Chitosan/Carboxymethyl Cellulose Composite Drug Coating

Yu-Zhu CHEN, Si-Si LIU, Meng-Meng ZHANG, Xiang-De LIN(), Dong-Dong ZENG()   

  1. School of Medical Devices,Shanghai University of Medicine & Health Science,Shanghai 201318,China
  • Received:2022-05-01 Accepted:2022-08-30 Published:2023-02-01 Online:2023-02-27
  • Contact: Xiang-De LIN,Dong-Dong ZENG
  • About author:zengdd@sumhs.edu.cn
    linxd@sumhs.edu.cn
  • Supported by:
    the Natural Science Foundation of Shanghai(22008151);the National Natural Science Foundation of China(19ZR1474300);the High-level Local University Construction Project of shanghai University of Medicine & Health Sciences(E1-2602-21-201006-1)

摘要:

以抗菌性壳聚糖和羧甲基纤维素为涂层主体材料,通过层层自组装成膜技术,利用抗菌性苦参碱为功能药物,构建了基于抗菌性壳聚糖/羧甲基纤维素复合药物涂层的聚氨酯敷料。再通过分子间化学交联改变了聚合物内部结构,提升涂层的机械稳定性。分别在硅片和聚氨酯敷料上成功制备了复合药物涂层。在模拟的生理条件下,分析了交联(CHI/CMC)25涂层、空白聚氨酯基底和交联(CHI/CMC)10涂层修饰的聚氨酯基底对苦参碱的药物装载和释放性能,三者的载药量分别为27.8、285.2和330.0 μg/cm2。相较于交联(CHI/CMC)25涂层和空白聚氨酯基底,聚氨酯基底(CHI/CMC)10交联涂层的载药量分别提升了10.9倍和20%,聚氨酯基底不仅具有良好的药物装载能力,还具有延缓药物释放速度的作用。交联(CHI/CMC)10涂层可以增强基底的力学性能,相较于原始的聚(对苯二甲酸乙二醇酯-1,4-环己二烯二亚甲基对苯二甲酸酯)(PETG)板,PETG板-交联(CHI/CMC)10涂层的断裂时间和标准应变均提升了25.1%。抑菌实验表明,由于壳聚糖和苦参碱的协同抗菌作用,与单药组相比,复合药物涂层的抑菌效果增强了1倍。复合药物涂层对金黄色葡萄球菌和大肠杆菌展现出明显的抑制生长作用,生长抑制率分别为35.7%和53.8%。

关键词: 聚氨酯敷料, 壳聚糖/羧甲基纤维素, 层层自组装, 复合药物涂层, 抗菌性

Abstract:

Using antibacterial chitosan and carboxymethyl cellulose as the main coating materials, the polyurethane dressing based on antibacterial chitosan/carboxymethyl cellulose composite drug coating is constructed by using antibacterial matrine as the functional drug through the layer-by-layer self-assembly membrane technology. Furthermore, the internal structure of the polymer is changed by intermolecular chemical cross-linking to improve the mechanical stability of the coating. Composite drug coatings are successfully prepared on silicon wafers and polyurethane dressings, respectively. The drug loading and release properties of crosslinked (CHI/CMC)25 coating, blank polyurethane substrate and crosslinked (CHI/CMC)10 coating modified polyurethane substrate are also analyzed under simulated physiological conditions for matrine with the drug loading of 27.8, 285.2 and 330.0 μg/cm2. Compared with the crosslinked (CHI/CMC)25 coating and the blank polyurethane substrate, the drug loading capacity of the polyurethane substrate (CHI/CMC)10 crosslinked coating is improved by 10.9 times and 20%, respectively. The polyurethane substrate not only has excellent drug loading capacity, but also has the effect of delaying drug release rate. The crosslinked (CHI/CMC)10 coating can enhance the mechanical properties of the substrate. Compared with the original poly(ethylene terephthalateco-1,4-cylclohexylen-edimethylene terephthalate (PETG) sheet, the fracture time and standard strain of the PETG sheet-crosslinked (CHI/CMC)10 coating are increased by 25.1%. Bacterial inhibition experiments show that the antibacterial effect of the compound drug coating is enhanced by 1-fold compared with the single drug group due to the synergistic antibacterial effect of chitosan and matrine. The compound drug coating shows significant growth inhibition against Staphylococcus aureus and Escherichia coli, with growth inhibition rates of 35.7% and 53.8%, respectively.

Key words: Polyurethane dressing, Chitosan/carboxymethyl cellulose, Layer-by-layer self-assembly, Composite drug coating, Antibacterial properties

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