有序大孔二氧化硅孔壁聚合物功能化修饰及葡萄糖淀粉酶固载化

doi:10.11944/j.issn.1000-0518.2018.11.170371

摘要/Abstract

摘要：

以丙烯酸为功能单体,甘油1,3-二甘油醇酸二丙烯酸酯作为交联剂,在孔内通过自由基引发聚合,成功地制备了在孔壁表面聚合物修饰的三维有序大孔氧化硅/聚合物复合材料(3DOM SiO₂-COOH)。通过拉曼光谱、扫描电子显微镜(SEM)、BET比表面测试和片剂硬度计技术手段表征了材料孔结构特征和机械强度。结果表明,3DOM SiO₂-COOH具有均匀的相互连接的大孔结构,孔壁表面形成了一层致密的11~32 nm厚度的聚合物膜,且具有较高的机械强度高。以3DOM SiO₂-COOH材料为载体,葡萄糖淀粉酶在其内固载能均匀地分布在材料内部。固载酶和游离酶的最佳反应pH均为5,最佳反应温度为55 ℃,米氏常数分别为3.78和3.97 g/L。固载酶具有更高的热稳定性、pH值稳定性、储藏稳定性和重复使用稳定性。 3DOM SiO₂-COOH材料可作为一种新型的固载酶载体。

关键词: 有序大孔, 表面改性, 复合材料, 固载酶

Abstract:

This paper presented a facile method to the modification of the three-dimensionally ordered macroporous(3DOM) SiO₂ hybrids with a carboxyl functionalized polymer layer on the macropore walls. Acrylic acid and glycerol 1,3-diglycerolate diacrylate were copolymerized to form the polymer layer in the macropores. Raman spectra, scanning electron microscopy(SEM) and BET measurements show that the prepared 3DOM SiO₂@polymer composites(SiO₂-COOH) have an uniform interconnected macroporous structure and the macropore walls are covered by a nanoscaled compact polymer layer. Moreover, the 3DOM SiO₂-COOH has an improved mechanical strength. The 3DOM SiO₂-COOH was further used as the support to immobilize glucoamylase. The results show that the immobilized enzyme homogeneously distributes in the 3DOM materials. The optimum pH of immobilized and free enzymes is both at 5, and the optimal reaction temperature is at 55 ℃. Michaelis constants of the immobilized enzyme and free enzyme are 3.78 g/L and 3.97 g/L, respectively. The immobilized enzyme presents higher thermal, pH, storage stabilities and higher reusability compared with the free enzyme. The results indicate that 3DOM SiO₂-COOH could be a novel support for the immobilization of enzymes.

Key words: ordered macropore, surface modification, composite, immobilized enzyme

叶丽芳,邬泉周. 有序大孔二氧化硅孔壁聚合物功能化修饰及葡萄糖淀粉酶固载化[J]. 应用化学, 2018, 35(11): 1309-1316.

YE Lifang,WU Quanzhou. Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls[J]. Chinese Journal of Applied Chemistry, 2018, 35(11): 1309-1316.

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