苏丹红Ⅰ印迹表面等离子共振传感器的制备与表征

doi:10.3724/SP.J.1095.2014.30577

摘要/Abstract

摘要： 以苏丹红I印迹的凝胶膜对模板分子的响应收缩为基础，利用表面引发聚合技术在表面等离子共振（SPR）芯片上制备了苏丹红I印迹SPR传感器，利用电化学和溶胀测量技术对传感器进行表征，建立了对苏丹红I的SPR检测方法。该方法的线性范围为8.0×10-10~1.0×10-8 mol/L(R2=0.9987)。方法用于样品红辣椒粉和腌制品中苏丹红I的检测，其检出限分别为3.4×10-10和2.5×10-10 mol/L。红辣椒粉中两个样品的相对标准偏差为1.38%和2.10%，腌制品试样的相对标准偏差为1.13%。说明该方法具有较高的灵敏度和较好的的重现性。

关键词: 分子印迹凝胶膜, 表面等离子共振传感器, 苏丹红Ⅰ

Abstract: Based on the Sudan Ⅰ-responsive shrinkage of the Sudan Ⅰ-imprinted gel film, a new imprinted gel-surface plasmon resonance(SPR) sensor for the detection of Sudan I was prepared. The Sudan I-imprinted soft gel film was synthesized on the surface of a SPR chip. The SPR sensor was characterized with an electrochemical method and swelling measurements. The method exhibits a linear response in the range of 8.0×10-10 to 1.0×10-8 mol/L(R2=0.9987) for the detection of Sudan Ⅰ. It was used to determine Sudan I in the paprika and salted products. The detection limits are 3.4×10-10 mol/L and 2.5×10-10 mol/L, respectively, and the relative standard deviations(RSD) are 1.38% and 2.10% for two paprika samples and 1.13% for the salted product. This SPR sensor shows high sensitivity and good reproducibility.

Key words: molecularly imprinted gel film, surface plasmon resonance sensor, Sudan Ⅰ

中图分类号:

O657

周春燕, 陈长宝, 李洁, 隋红光, 高吉刚, 周杰. 苏丹红Ⅰ印迹表面等离子共振传感器的制备与表征[J]. 应用化学, DOI: 10.3724/SP.J.1095.2014.30577.

ZHOU Chunyan, CHEN Changbao, LI Jie, SUI Hongguang, GAO Jigang*, ZHOU Jie*. Preparation and Characterization of Sudan Ⅰ-imprinted Surface Plasmon Resonance Sensor[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30577.

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