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3,5-二碘水杨醛缩邻苯二胺Ni(Ⅱ)配合物的合成及生物活性

李晓红,杨天林*,王敏,霍丽娜,李艳萍   

  1. (宁夏大学化学化工学院能源化工重点实验室 银川 750021)
  • 收稿日期:2012-01-13 修回日期:2012-02-28 出版日期:2013-01-10 发布日期:2013-01-10
  • 通讯作者: 杨天林，教授; Tel:0951-2061004; Fax:0951-2062860; E-mail:yang_tl@nxu.edu.cn; 研究方向：稀土配位化学
  • 基金资助:
    国家自然科学基金（20962016）教育部科学技术研究重点基金 (208159) 资助项目

Synthesis and Biological Activities of 3,5-Diiodo-salicylalidehyde and o-Diaminobenzene Schiff Base and Its Ni(Ⅱ) Complex

LI Xiaohong, YANG Tianlin*, WANG Min, HUO Lina, LI Yanping   

  1. (Key Laboratory of Energy Sources and Chemical Engineering,College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China)
  • Received:2012-01-13 Revised:2012-02-28 Published:2013-01-10 Online:2013-01-10
  • Contact: yang

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被引次数

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摘要： 合成了3,5-二碘水杨醛缩邻苯二胺席夫碱合镍(Ⅱ)配合物。 通过核磁共振、元素分析、紫外光谱、红外光谱及摩尔电导对结构进行了表征，用Gaussian03程序优化计算，确定配合物的结构为Ni(Ⅱ)L。 通过紫外光谱、粘度法及与溴化乙锭(EB)的竞争实验，研究了配合物与小牛胸腺DNA(ct-DNA)的作用情况。 结果显示，配合物与DNA作用时，紫外吸收发生明显的减色效应，其结合常数为Kb=1.129×105 L/mol；EB-DNA体系的荧光强度随配合物的加入迅速减弱；配合物的加入使ct-DNA的粘度增加。 这些结果表明，该化合物以插入式与ct-DNA键合。 并用打孔法测试了配合物对藤黄微球菌(M.luteus)的抑制作用。

关键词: 含碘席夫碱, Ni (Ⅱ)配合物, 光谱性质, DNA插入作用, 抑菌活性

Abstract: The Schiff base consisted of 3,5-diiodo-salicylalidehyde and o-diaminobenzene(L) and its Ni(Ⅱ) metal complex has been synthesized and characterized by 1H NMR, elemental analysis, UV spectra, IR spectra and molar conductance analysis. The main bond parameters of the complex were calculated by full-optimized molecular geometry using Gaussian03 program. The composition of the complex was determined to be Ni(Ⅱ)L. The binding mode between complex and DNA was investigated by UV spectra, viscosities, and DNA competitive binding with ethidium bromide(EB). The results showed that the intensity of absorption peaks increased when the complex interacted with ct-DNA, and its constant was Kb=1.129×105 L/mol. The complex will lead to dramatic decrease of fluorescent intensity of EB-NA. The relative viscosity of ct-DNA increased with the concentration increase of the complex. All indicated that the complex was intercalated into ct-DNA. In addition, the complex is assayed against M.luteus by the punch method.

Key words: Iodine-containing Schiff base, Ni(Ⅱ) complex, spectrum character, DNA Intercalative binding mode, antibacterial activity

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