应用化学 ›› 2011, Vol. 28 ›› Issue (05): 602-604.DOI: 10.3724/SP.J.1095.2011.00430

• 研究简报 • 上一篇    下一篇

离子液体对淀粉酶催化反应的影响

于游*,聂毅   

  1. (曲阜师范大学化学与化工学院 曲阜 273165)
  • 收稿日期:2010-07-29 修回日期:2010-09-13 出版日期:2011-05-10 发布日期:2011-05-10
  • 通讯作者: 于游,讲师; Tel:0537-4456301; Fax:0537-4458187; E-mail:yuyou@mail.qfnu.edu.cn; 研究方向:生物热动力学
  • 基金资助:
    山东省自然科学基金(ZR2009BM035)和山东省博士基金(2008BS02021)资助项目

The Effects of Ionic Liquids on the Catalytic Reaction of Amylase

YU You*, NIE Yi   

  1. (College of Chemistry and Chemical Engineering,Qufu Normal University,Qufu 273165)
  • Received:2010-07-29 Revised:2010-09-13 Published:2011-05-10 Online:2011-05-10

摘要:

用分光光度法研究了二烷基咪唑氯类和二烷基咪唑磷酸酯类离子液体对淀粉酶催化反应的影响,通过吸光度的变化来测定淀粉水解的程度。 结果表明,这两类离子液体对淀粉酶催化反应均有明显的抑制作用,其中1-己基-3-甲基咪唑氯[HMIM]Cl的抑制作用最大。 而且,对于相同的阴离子,随着阳离子咪唑环烷基取代基链长的增加,抑制作用也增加。

关键词: 离子液体, 淀粉酶催化, 分光光度法, 抑制

Abstract:

The effects of N,N-dialkylimidazolium-based ionic liquids on the catalytic reaction of amylase were studied in this paper via the spectroscopy method. The results indicate that the ionic liquids show inhibitory activities on the catalytic reaction of amylase. 1-Butyl-3-methylimidazolium chloride([HMIM]Cl) showed the highest inhibitory activity among all investigated ionic liquids in this study. Furthermore, the inhibition effect of ionic liquids having the same anion increases with the alkyl group length of the imidazolium ring.

Key words: ionic liquid, amylase catalyzing, spectrophotometry, inhibition

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