中华医学超声杂志

应用化学 ›› 2009, Vol. 26 ›› Issue (09): 1088-1089.

• 研究论文 • 上一篇    下一篇

用磷酸二异辛酯/正辛醇反胶束从面包酵母粗提液中萃取谷胱甘肽

周小华1*,王东2,郑声申1   

  1. (1.重庆大学化学化工学院 重庆 400030；2.重庆市固体废物管理中心 重庆)
  • 收稿日期:2008-09-10 修回日期:2009-02-16 出版日期:2009-09-10 发布日期:2009-09-10
  • 通讯作者: 周小华，男，教授; E-mail:zhou65306590@ tom.com; 研究方向：生物化工及药物化学

Extraction of glutathione with reversed micelles

ZHOU Xiao-Hua1*, WANG Dong2, ZHENG Qing-Shen1   

  1. (1.College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400030;
    2.Chongqing Solid Waste Management Center,Chongqing)
  • Received:2008-09-10 Revised:2009-02-16 Published:2009-09-10 Online:2009-09-10

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1516

被引次数

4

摘要：

采用磷酸二异辛酯（P204）/正辛醇反胶束液从面包酵母粗提液中萃取还原型谷胱甘肽(GSH)，研究了各种因素对萃取率的影响以及水相pH值和P204浓度与GSH萃取率关系的数学模型。正交实验确定，GSH的最佳萃取条件是水相pH值2.0，K+浓度0.1 mol•L-1，萃取时间15 min，P204浓度0.16 mol•L-1，GSH单次的萃取率为33.1%，2次萃取率达到49.7%。

关键词: 面包酵母, 谷胱甘肽, 反胶束萃取, P204/正辛醇反胶束

Abstract:

he extraction of glutathione (GSH) from distillate of bread yeast with reversed micelles made up of phosphoric acid dioctyl ester (P204) and n-octanol was researched. The effect factors on Extraction rate of GSH were examined. The optimum conditions were confirmed in the orthogonal tests that extraction time is 15 min; pH value in aqueous phase is 2.0; concentration of P204 is 0.16 mol•L-1 and concentration of K+ is 0.1 mol•L-1. The extraction rate of GSH is 33.1% and the twi-extraction rate got 49.7% in the optimum conditions.

Key words: Bread yeast, Glutathione (GSH), Extraction with reversed micelles, reversed micelles of P204 / octanol

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