中华医学超声杂志

应用化学 ›› 1997, Vol. 0 ›› Issue (3): 33-36.

• 研究论文 • 上一篇    下一篇

增效试剂对过氧化物模拟酶催化显色体系的增效作用——Fe-TPPS4-4-AAP-苯酚衍生物-H2O2体系

苏小笛1, 刘昭林1, 朱敏2, 沈含熙2   

  1. 1. 天津大学应用化学系, 天津 300072;
    2. 南开大学化学系, 天津
  • 收稿日期:1996-08-27 修回日期:1997-01-21 出版日期:1997-06-10 发布日期:1997-06-10

Enhanced Effect of Enhancing Agents on the Oxidation Reaction Catalyzed by Mimetic Horseradish Peroxidase ——Fe-TPPS4-4-AAP-Phenol-H2O2 System

Su Xiaodi1, Liu Zhaolin1, Zhu Min2, Shen Hanxi2   

  1. 1. Department of Applied Chemistry, Tianjin University, Tianjin 300071;
    2. Department of Chemistry, Nankai University, Tianjin
  • Received:1996-08-27 Revised:1997-01-21 Published:1997-06-10 Online:1997-06-10

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摘要： 本文以β-环糊精(β-CD)和表面活性剂为增效试剂,分别研究了它们对以Fe-meso-(四(4-磺基苯)卟啉)(Fe-TPPS4)为催化剂,催化过氧化氢氧化4-氨基安替比林(4-AAP)与苯酚衍生物显色反应的速度和灵敏度的影响,发现β-CD和十二烷基苯磺酸钠(SDBS)对该体系具有明显的增效作用。在3×10-3mol/Lβ-CD存在下,测定H2O2的灵敏度比文献报道的提高了1.56倍。利用SDBS浓度与催化显色反应初速度的增加值之间的线性关系,建立了测定微量SDBS的方法

关键词: β－环糊清, 表面活性剂, 过氧化物模拟酶, H2O2的测定, 增效分光光度法

Abstract: The enhanced effect of β-cyclodextrin(β-CD)or surfactants on the oxidation reaction of H2O2 with 4-aminoantipyrine and phenol, using iron-(4-sulphophenyl)porphine(Fe-TPPS4)as catalyst has been studied. It is indicated that the initial rate and sensitivity of this color reaction are increased with the added amount of β-CD or sodium dodecylbenzene sulfate(SDBS). A sensitive method for the determination of H2O2 in the presence of β-CD has been developed. Trace amount of SDBS can be determined based on the linear relationship between the increment of the reaction rate and the concentration of the surfactant.

Key words: β-cyclodextrin, surfactant, mimetic enzyme, H2O2 determination, enhanced spectrophotometry

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