应用化学 ›› 1995, Vol. 0 ›› Issue (1): 108-110.

• 研究论文 • 上一篇    下一篇

葡萄糖氧化酶电极的制备

于秀娟, 周定, 郭京华   

  1. 哈尔滨工业大学应用化学系, 哈尔滨 150006
  • 收稿日期:1994-01-24 修回日期:1994-06-07 出版日期:1995-02-10 发布日期:1995-02-10

Preparation of Glucose Oxidase Electrode

Yu Xiujuan, Zhou Ding, Guo Jinghua   

  1. Department of Applied Chemistry, Harbin Institute of Technology, Harbin 150001
  • Received:1994-01-24 Revised:1994-06-07 Published:1995-02-10 Online:1995-02-10

摘要: 酶与电极的连接即酶膜的形成是制备酶电极的关键步骤之一,有许多种方式.葡萄糖氧化酶(GOD)电极是研究较早和报道较多的一类酶电极.本文采用吸附法和共价结合法制 备了3类葡萄糖氧化酶(GOD)电极,研究了酶膜与酶电极响应特性之间的关系,比较了酶膜 厚度、固定化酶的含量以及酶膜的形成方法等因素对GOD电极的响应时间、灵敏度、线性检 测范围等一些响应特性的影响.实验结果表明,酶电极的响应特性主要取决于酶膜的形成方法,酶膜内固定酶量的增加有利于扩大酶电极的线性检测范围,酶膜的厚度要适宜,以便使酶膜保持一定的透过性,从而得到响应快、灵敏度高的酶电极.为优化酶电极设计提供依据.

关键词: 葡萄糖氢化酶, 酶电极, 制备

Abstract: Three types of glucose oxidase (GOD) electrode were fabricatpd by absorbing and/or cross-linking. It was found that the response properties of the enzyme electrode weremainly determined by the methods of membrane formation. In order to obtain an enzyme electrode having fast response time and large current values,a suitable thickness of enzyme membrane is required.

Key words: glucose oxidase, enzyme electrode, preparation