应用化学 ›› 1992, Vol. 0 ›› Issue (2): 106-110.

• 研究简报 • 上一篇    下一篇

双羟基极性键合固定相在氨基酸分析上的应用

达世禄, 刘武平   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1991-02-25 修回日期:1991-08-22 出版日期:1992-04-10 发布日期:1992-04-10
  • 基金资助:
    国家自然科学基金

A GLYCEROLPROPYL-DIETHYLENESILYL BONDED PHASE FOR AMINO ACID SEPARATION AND DETERMINATION

Da Shilu, Liu Wuping   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1991-02-25 Revised:1991-08-22 Published:1992-04-10 Online:1992-04-10

摘要: 我们曾报道了一类新型双羟基键合固定相(GDSB)的合成[1],研究表明这类固定相具有键合相浓度高,热和化学稳定性好的特点,可用于性质相似的有机化合物的分离。本文以GDSB为色谱柱填料,含Cu2+离子和烷基磺酸盐的HAc-NaAc缓冲溶液作流动相,结合紫外检测,分离测定了氨基酸。

关键词: 键合固定相, 氨基酸, 色谱分析

Abstract: Separation and determination of amino acids were carried out by means of a glycerolpropyl-diethylenesilyl bonded phase (GDSB) combined with a UV-detector at 254nm. An acetate buffer solution (pH 5.75) containing 2.5?10-4 mol/L of copper ion and 2.5?10-4 mol/L of hexyl sulfonate was used as a mobile phase. The effects of the mobile phase composition on capacity factor and detection sensitivity were examined. The results the suifability of GDSB system as a stationary phase for analysis of amino acids showed by HPLC.

Key words: glycerolpropyl-diethylenesilyl bonded phase, Amino acid, chromatographic analysis