中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (03): 349-354.DOI: 10.3724/SP.J.1095.2011.00210

• 研究论文 • 上一篇    下一篇

基于血红蛋白/纳米金/二氧化锆-壳聚糖修饰的过氧化氢生物传感器

邓祥*,黄小梅   

  1. (四川文理学院化学与化学工程系 达州  635000)
  • 收稿日期:2010-04-12 修回日期:2010-06-02 出版日期:2011-03-10 发布日期:2011-03-10
  • 通讯作者: 邓祥，讲师; Tel/Fax:0818-2760749; E-mail:dxw8066031@163.com; 研究方向：电化学分析
  • 基金资助:
    四川省教育厅重点项目（10ZA143），四川省教育厅青年基金项目（10ZB105）

A Biosensor for Hydrogen Peroxide Based on Hemoglobin Peroxidase Immobilized on nano-Au/Chitosan-ZrO2/gold Electrode

DENG Xiang*, HUANG Xiaomei   

  1. (Department of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou 635000)
  • Received:2010-04-12 Revised:2010-06-02 Published:2011-03-10 Online:2011-03-10
  • Contact: Deng Xiang

PDF

1971

被引次数

3 | 2

摘要：

将二氧化锆(ZrO2)分散于壳聚糖(CS)中得到稳定的CS-ZrO2复合物，制备了Hb/nano-Au/CS-ZrO2/Au过氧化氢生物传感器。 用循环伏安法和计时电流法考察该修饰电极的电化学特性，发现该修饰电极对过氧化氢(H2O2)的还原有良好的电催化作用。 实验结果表明，该传感器对H2O2的线性响应范围为3.9×10-6～1.8×10-3 mol/L，线性相关系数R=0.9956，检测下限为5.6×10-7 mol/L(S/N=3)，并具有选择性高、线性范围宽和响应快等优点。

关键词: 二氧化锆, 壳聚糖, 纳米金, 血红蛋白

Abstract:

A new approach to measure with hemoglobin(Hb)/nano-Au/chitosan(CS)-ZrO2/Au-modified electrode was established. Zirconium dioxide(ZrO2) was dispersed in the chitosan(CS) to form a stable CS-ZrO2 composite. This composite was first coated on the surface of gold electrode to provide an amino group interface for assembling nano-Au, followed by the adsorption of Hb to form a bilayer of Hb/nano-Au. The Hb/nano-Au/CS-ZrO2/gold electrode was used as hydrogen peroxide (H2O2) sensor. Cycling voltammetric and chronoamperometric characterizations reveal that the modified electrode exhibit electrocatalytic behavior to H2O2. The results confirmed that the electrode exhibited high sensitivity, fast response, and linear response in the range of 3.9×10-6~1.8×10-3 mol/L(R=0.9956) with the lowest detection limit of 5.6×10-7 mol/L(S/N=3).

Key words: Zirconium dioxide (ZrO2), Chitosan, nano-Au, Hemoglobin

中图分类号: 

网站版权 © 应用化学     本网站备案：吉ICP备12000082号