中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (10): 1209-1214.DOI: 10.3724/SP.J.1095.2010.90828

• 研究论文 • 上一篇    下一篇

液质联用技术研究人参与干姜或赤芍配伍前后人参皂苷及其抗氧化活性的变化

杜芹芹1,2,张旭1,宋凤瑞1,刘志强1,刘淑莹1*   

  1. (1.中国科学院长春应用化学研究所,长春质谱中心 长春 130022;
    2.中国科学院研究生院 北京)
  • 收稿日期:2009-12-09 修回日期:2010-03-08 出版日期:2010-10-10 发布日期:2010-10-10
  • 通讯作者: 刘淑莹，女，博士，研究员; E-mail:mslab@ciac.jl.cn; 研究方向：有机质谱
  • 基金资助:
    国家支撑计划（2007BAI38B04-03）和吉林省科技发展重大及重点（20086043,20090908）资助项目

The Application of HPLC-ESI-MS or FRAP in Studying the Variation of Ginsenosides and Their Anti-oxidation after the Combination of Ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra

DU Qin-Qin1,2, ZHANG Xu1, SONG Feng-Rui1, LIU Zhi-Qiang1, LIU Shu-Ying1*   

  1. (1.Changchun Center of Mass Spectrometry,Changchun Institute of Applied Chemistry,
    Chinese Academy of Sciences,Changchun 130022;
    2.Graduate School of Chinese Academy of Sciences,Beijing)
  • Received:2009-12-09 Revised:2010-03-08 Published:2010-10-10 Online:2010-10-10

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1470

被引次数

8 | 1

摘要：

采用高效液相色谱与电喷雾质谱联用技术(HPLC-ESI-MS)，对不同质量比的人参与干姜或赤芍配伍过程中人参皂苷的变化进行了研究，发现随加入的干姜量增加，共煎液中的人参皂苷含量依次降低；少量的赤芍可以使各皂苷的溶出量增加；同时测定了人参单煎液、人参与干姜、赤芍共煎液中正丁醇提取物和水提物的抗氧化活性。 以抗坏血酸(500 μmol/L)作对照，人参与干姜、赤芍配伍溶液的抗氧化活性比人参单煎液要好，同时人参与干姜、赤芍共煎液中正丁醇提取物的FRAP(铁离子还原/抗氧化能力测定)值分别为1562.29和2969.78 μmol/L，高于人参与2种药单煎液（1260.27和2502.07 μmol/L）之和。

关键词: 人参, 干姜, 赤芍, 配伍, 抗氧化

Abstract:

To study the content variation of ginsenosides after the combination of ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra, a high performance liquid chromatography-electrospray ionization spectrometric(HPLC-ESI-MS) method was used to analyze and compare the ginsenosides in the decoction. The experimental results show that the contents of ginsenosides decreased along with the increased amount of Zingiber officinale Rosc, while the stripping of ginsenosides was promoted by a small amount of Radix Paeoniae Rubra. In addition, the total antioxidant activity of n-butanol and water extracts from the single and compatible decoctions of ginseng was also determined by ferric reducing antioxidant power(FRAP) method. With ascorbic acid(500 μmol/L) as control, the anti-oxidation of compatible decoction of ginseng with Zingiber officinale Rosc/Radix Paeoniae Rubra was higher than that of the single decoction of ginseng; and the FRAP values of n-butanol extracts from the compatible decoction of ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra were 1562.29 and 2969.78 μmol/L, respectively, greater than the sum of single decoction of ginseng with Zingiber officinale Rosc(1260.27 μmol/L)/Radix Paeoniae Rubra(2502.07 μmol/L), indicating that the antioxidant activity of n-butanol extract from compatible decoction was better.

Key words: ginseng, Zingiber officinale Rosc, Radix Paeoniae Rubra, combination, antioxidant activity

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