脐橙果皮的室温和热辅助表面解吸常压化学电离质谱的比较

doi:10.11944/j.issn.1000-0518.2015.10.150092

摘要/Abstract

摘要：

以脐橙果皮为研究对象,比较了室温和热辅助表面解吸常压化学电离源(DAPCI)的解吸电离特性,结合串联质谱技术,对果皮中香气、糖、黄酮类等主要成分进行了鉴定,并通过主成分分析(PCA)方法对热辅助DAPCI-MS所获外果皮指纹谱图信息进行分析,进而区分了3个不同产地脐橙。结果表明,室温DAPCI-MS对脐橙果皮中的香气成分具有较强的解吸电离能力,而热辅助DAPCI-MS则更适合于分析果皮中的黄酮类和糖类及其衍生物。 DAPCI-MS具有灵敏度高、分析速度快等特点,有望成为农产品品质快速检测与产地识别研究的新方法。

关键词: 表面解吸常压化学电离源, 质谱, 主成分分析方法, 脐橙

Abstract:

Comparison between ambient temperature and thermal surface desorption atmospheric pressure chemical ionization mass spectrometry(DAPCI-MS) was made with pericarp of navel orange. The main components including aroma compounds, flavanones and sugars were identified by tandem mass spectrometry. Following the method of thermal DAPCI-MS, the mass spectra of epicarp were obtained and were further analyzed by principle component analysis(PCA), which allows a confident discrimination of navel orange samples from three different origins. The results show that aroma compounds are desorbed effectively by ambient temperature DAPCI-MS, while flavanones, sugars and its derivatives are analyzed easily by thermal DAPCI-MS. DAPCI-MS is attractive by showing the advantage of high sensitivity and analytical speed, and thus provides a possible new method to rapidly screen the quality of agricultural products and distinguish origins.

Key words: surface desorption atmospheric pressure chemical ionization, mass spectrometry, principal component analysis, navel orange

方小伟,钟涛,姚国灿,高翔,杨美玲,李慧,乐长高,张兴磊. 脐橙果皮的室温和热辅助表面解吸常压化学电离质谱的比较[J]. 应用化学, 2015, 32(10): 1201-1207.

FANG Xiaowei,ZHONG Tao,YAO Guocan,GAO Xiang,YANG Meiling,LI Hui,LE Zhanggao,ZHANG Xinglei. Comparison of Ambient Temperature and Thermal Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectra for the Pericarp of Navel Orange[J]. Chinese Journal of Applied Chemistry, 2015, 32(10): 1201-1207.

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