Preparation and Properties of Antibacterial Composite Films with High Loading of Citral

doi:10.19894/j.issn.1000-0518.240033

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (9): 1284-1296.DOI: 10.19894/j.issn.1000-0518.240033

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Preparation and Properties of Antibacterial Composite Films with High Loading of Citral

Tian-He GAO, Li-Juan YAN, Ming-Qing CHEN, Wei-Fu DONG, Dong-Jian SHI()

The Key Laboratory of Synthetic and Biological Colloids，Ministry of Education，School of Chemical and Material Engineering，Jiangnan University，Wuxi 214122，China

Received:2024-01-31 Accepted:2024-05-20 Published:2024-09-01 Online:2024-10-09
Contact: Dong-Jian SHI
About author:djshi@jiangnan.edu.cn
Supported by:
the National Natural Science Foundation of China(52103165)

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Abstract

Abstract:

The use of antibacterial packaging materials is an effective way to delay fruit rotting and spoilage， thereby minimizing economic losses and health hazards. Natural antibacterial agents， especially essential oils， are often selected as antibacterial components in antibacterial packaging materials， due to their excellent antibacterial properties. However， their low water solubility， poor stability， and easy volatility result in materials containing them having poor mechanical properties and short antibacterial efficacy time. Therefore， in this work， the citral loaded Pickering emulsion was prepared using chitosan and self-made carboxymethyl glucan self-assembled nanoparticles as emulsifier. Then， composite films with good transparency， excellent mechanical properties and long-term antibacterial properties for fruits packaging were prepared from chitosan （CS）， carboxymethyl-glucan （CMG）， poly（vinyl alcohol）（PVA）， and Pickering emulsion （PE） by solvent casting strategy. Pickering emulsion has a protective effect on citral， which not only improves the compatibility of citral in composite film substrate， but also extends the antibacterial time of packaging materials. In this experiment， the transparency of the antibacterial composite film containing Pickering emulsion （CS-CMG-PVA-PE1） was 84%， the tensile strength was 32 MPa， and the elongation at break was 130%. More importantly， the composite polymer solution can be dipped directly on fruits as a coating for food storage to improve food shelf life， substantially expanding its ease of use and scope of use.

Key words: Pickering emulsion, Citral, Self-assembling nanoparticles, Antibacterial properties

CLC Number:

O636

Tian-He GAO, Li-Juan YAN, Ming-Qing CHEN, Wei-Fu DONG, Dong-Jian SHI. Preparation and Properties of Antibacterial Composite Films with High Loading of Citral[J]. Chinese Journal of Applied Chemistry, 2024, 41(9): 1284-1296.

Figures/Tables 12

Fig.1 （A） FT-IR spectra of Dex and HCMG，（B） GPC curve of HCMG and （C） display diagrams of self-assembly between CS and CMGs with different molecular mass

Fig.2 Schematic illustration of the construction mechanism of CS and HCMG self-assembled nanoparticles and the formation diagram of Pickering emulsion

Fig.3 Photos of self-assembly status of CS and HCMG with different mass ratios

Table 1 Particle size and distribution of CS and HCMG self-assembled nanoparticles with different mass ratios

Sample	m（CS）∶m（HCMG）	Average particle size/nm	PDI
1	20∶1	981.2±15.23 ^a	0.334±0.047 ^b
2	10∶1	493.5±12.25 ^b	0.325±0.063 ^b
3	5∶1	480.5±11.32 ^b	0.286±0.054 ^b
4	2∶1	421.1±9.56 ^c	0.295±0.046 ^b
5	1∶1	376.8±8.71 ^d	0.104±0.032 ^d
6	1∶2	333.3±8.35 ^e	0.546±0.081 ^a

Fig.4 Superdepth microscope images of Pickering emulsion for encapsulated citral by self-assembled CS and HCMG nanoparticles with different mass ratios

Table 2 Loading efficiency of citral by self-assembled CS and HCMG nanoparticles with different mass ratios and size and distribution of Pickering emulsion

m（CS）∶m（HCMG）	Average particle size/μm	PDI	Loading efficiency/%
20∶1	2.878±0.205 ^a	0.778±0.089 ^a	10.5
10∶1	2.547±0.178 ^b	0.635±0.025 ^b	13.5
5∶1	1.945±0.123 ^c	0.574±0.065 ^c	18.5
2∶1	2.0001±0.157 ^c	0.612±0.086 ^c	65.1
1∶1	2.05±0.167 ^c	0.624±0.035 ^c	22.1
1∶2	- ^d	- ^d	6.5

Fig.5 （A-D） Digital photographs，（A′-D′） surface SEM images， and （A″-D″） cross-sectional SEM images of the CS-CMG-PVA， CS-CMG-PVA-PE1， CS-CMG-PVA-PE2， and CS-CMG-PVA-PE3 composite films （all the films are 90 mm in diameter）

Fig.6 （A） FT-IR spectra，（B） enlarged image of ATR-FTIR spectra，（C） XRD patterns and （D） UV-Vis transmittance of all the prepared composite films

Fig.7 （A） Stretch curves，（B） elastic modulus and fracture toughness and （C） TGA analysis of all prepared composite films

Fig.8 Citral release profile of films （Free Citral curve is the release of citral in the CS-CMG-PVA-Free composite film； Citral of PE curve represents the release of citral in CS-CMG-PVA-PE1 composite film）

Fig.9 （A） Photos of bacterial growth，（B） bacterial relative viability after coculturing with different composite films，（C） photos of bacterial growth and （D） bacterial relative viability after coculturing with CS-CMG-PVA-PE1 composite films for different times （P＜0.01）

Fig.10 （A） Growth status of strawberries coated with different coatings，（B） mass loss and （C） changes in firmness

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Preparation and Properties of Antibacterial Composite Films with High Loading of Citral

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