Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (5): 677-686.DOI: 10.19894/j.issn.1000-0518.230305
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Feng ZHOU1,2, Xiao-Qing CAI1,2,3(), Qiao-Wei TANG5, Ying ZHU3,4, Li-Hua WANG3,4, Jun HU3,4
Received:
2023-10-05
Accepted:
2024-04-05
Published:
2024-05-01
Online:
2024-06-03
Contact:
Xiao-Qing CAI
About author:
caixq@sari.ac.cnSupported by:
CLC Number:
Feng ZHOU, Xiao-Qing CAI, Qiao-Wei TANG, Ying ZHU, Li-Hua WANG, Jun HU. Silver-Stained Golgi Staining for Imaging the Intact Structure of Neurons[J]. Chinese Journal of Applied Chemistry, 2024, 41(5): 677-686.
Fig.3 Optical microscopy imaging of K2Cr2O7 stained with different mass concentrations K2Cr2O7. K2Cr2O7 mass concentrations of (A) 20 mg/mL, (B) 40 mg/mL, (C) 60 mg/mL and (D) 80 mg/mL, (E) Sholl analysis of neuronal morphology, red oval dashed box: black deposits, blue oval dashed box: directional nerve fibers, red rectangular dashed box: neuronal structures with soma and dendritic structures, blue rectangular dashed box: vascular structures
Fig. 4 Optical microscope imaging of the tissue under different staining time ratioPFA mixed solution with K2Cr2O7 and K2Cr2O7 solution staining time ratios were 0∶4 (A), 1∶3 (B), 2∶2 (C), 3∶1 (D), 4∶0 (E), (F) Sholl analysis of neuronal morphology, red oval dashed box: black deposits, blue oval dashed box: incomplete neuronal structures, red rectangular dashed box: neuronal structures with soma and dendritic structures, blue rectangular dashed box: vascular structures
Fig.5 Optical microscopy imaging of stained with different mass concentrations AgNO3. AgNO3 mass concentrations of 5 (A), 7.5 (B), 10 (C), 12.5 (D), 15 (E) and 20 mg/mL (F), (G) Sholl analysis of neuronal morphology, red oval dashed box: black deposits, blue oval dashed box: incomplete neuronal structures, red rectangular dashed box: neuronal structures with soma and dendritic structures, blue rectangular dashed box: vascular structures
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