A Visual Semi⁃quantitative Method for Rapid Detection of Copper Ion in Water

doi:10.19894/j.issn.1000-0518.210399

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (8): 1303-1311.DOI: 10.19894/j.issn.1000-0518.210399

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A Visual Semi⁃quantitative Method for Rapid Detection of Copper Ion in Water

Feng-Zhou XU¹^,²^,³(), Hua-Ying TANG¹, Wu-Hui LIU¹, Yi-Feng JIANG¹, Wen-Kai LI¹, Xian-Hai LU¹

^1.College of Environmental and Biological Engineering，Putian University，Putian 351100，China
^2.Fujian Provincial Key Laboratory of Ecology-toxicological Effects & Control for Emerging Contaminants，Putian University，Putian 351100，China
^3.Key Laboratory of Ecological Environment and Information Atlas，Fujian Province University，Putian University，Putian 351100，China

Received:2021-08-09 Accepted:2021-11-15 Published:2022-08-01 Online:2022-08-04
Contact: Feng-Zhou XU
About author:xufengzhou520@163.com
Supported by:
the Natural Science Foundation of Fujian Province(2020J05209);the Education and Scientific Research Project for Young and Middle?aged Teachers of Fujian Province(JAT190569);the Scientific Research Start?up Project of Putian University(2019116);the University Students Innovation and Entrepreneurship Training Plan of Fujian(S202011498013)

1. A Visual Semi-quantitative Method for Rapid Detection of Copper Ion in Water.pdf(866KB)

Abstract

Abstract:

Copper is one of the heavy metal pollutants. The development of point of care testing （POCT） is necessary in the emergency detection of pollution events， which can avoid the spread of the contaminated area. Based on DNA-stabilized fluorescent copper nanoparticles （CuNPs）， a ultraviolet（UV）-assisted POCT （fluorescence colorimetric） was developed for detection of Cu²⁺. In this method， after optimization of conditions， CuNPs were synthesized in the MOPS solution using 600 nmol/L poly（AT-TA） dsDNA as the scaffold and 2 mmol/L sodium ascorbate as the reducing agent. With the aid of a UV flashlight， the red-orange fluorescence of CuNPs is visible to naked-eye （wearing anti-UV goggles）. Also， the fluorescence brightness varies with the concentration of Cu²⁺. Based on this strategy， a semi-quantitative detection method for Cu²⁺ was developed， i.e.， the concentration range of Cu²⁺ in the water-sample could be detected by fluorescent colorimetry with standard series. The LOD of the method is 1.5 mg/L （24 μmol/L）， which meets the requirements of third-level of discharge standard in the Comprehensive Sewage Discharge Standard （GB 8978-1996）. This method is a new semi-quantitative， visual point of care testing for Cu²⁺， which can provide a reference method for rapid on-site detection of copper pollution in environmental water.

Key words: Copper ions, Copper nanoparticles, Fluorescence colorimetry, Visualization

CLC Number:

O661.1

Feng-Zhou XU, Hua-Ying TANG, Wu-Hui LIU, Yi-Feng JIANG, Wen-Kai LI, Xian-Hai LU. A Visual Semi⁃quantitative Method for Rapid Detection of Copper Ion in Water[J]. Chinese Journal of Applied Chemistry, 2022, 39(8): 1303-1311.

Figures/Tables 8

Table 1 DNA sequence used in this work

DNA	Sequence （5'→3'）
dsDNA	GTAGTCGTGATGAACGTATGAGCGTATGAGTATAGCTTAT
dsDNA	ATAAGCTATACTCATACGCTCATACGTTCATCACGACTAC
Poly （AT?TA）	ATATATATATATATATATATATATATATATATATATATAT）
Poly T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

Fig.1 （A） Schematic of DNA-templated CuNPs；（B） Schematic of Cu2+ detection based on CuNPs

Fig.2 Fluorescence spectra （A） and fluorescent photos （B） of the CuNPs templated by different DNAs under the UV excitation

Fig.3 Fluorescence spectra （A） and fluorescent photos （B） of CuNPs under different concentrations of DNA under the UV excitation

Fig.4 Fluorescence spectra （A） of different CuNPs samples prepared under different concentrations of sodium ascorbate and fluorescent photos of the corresponding CuNPs samples under the UV excitation 0 min （B） and 15 min （C）

Fig.5 Assay specificity and anti-interference investigation of this method(A) Fluorescence response of CuNPs to various metal ions (except for samples Cu2+ and mix, all other samples have no fluorescence signal); (B) Fluorescent photos of the corresponding CuNPs samples under the UV excitation

Fig.6 Fluorescent colorimetric standard series of CuNPs under UV excitation

Fig.7 Fluorescent photos of the copper pollution water sample detection under UV excitation

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A Visual Semi⁃quantitative Method for Rapid Detection of Copper Ion in Water

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