Electrospray Ionization Tandem Mass Spectrometry on the Interaction of Baicalin and Glutathione

doi:10.19894/j.issn.1000-0518.200386

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 331-342.DOI: 10.19894/j.issn.1000-0518.200386

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Electrospray Ionization Tandem Mass Spectrometry on the Interaction of Baicalin and Glutathione

TANG Jun¹, DU Yang^1,2, ZHAO Feng-Jiao^1,2, CUI Meng^1,2*, LIU Zhi-Qiang^1,2

¹National Center for Mass Spectrometry in Changchun, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
²University of Science and Technology of China, Hefei 230029, China

Received:2020-12-21 Accepted:2021-02-05 Published:2021-03-01 Online:2021-05-01
Supported by:
National Natural Science Foundation of China (No.21675150)

Abstract

Abstract: The interaction between baicalin and glutathione was studied by electrospray ionization multiple-stage tandem mass spectrometry. The results show that the oxidation product of baicalin can interact with glutathione. Adduct of glutathione and baicalin is unstable and further undergoes reactions such as auto-oxidation, hydration or deuronic acid. The binding site of baicalin on glutathione is identified at the C8 position of baicalin aglycone. These results provide insights of the interaction of glutathione with baicalin at molecular level without the need of the complicated sample separation and purification processes. This strategy can be applied to investigate the interaction of other natural products and peptides.

Key words: Electrospray ionization mass spectrometry, Interaction, Baicalin, Glutathione

CLC Number:

O657

TANG Jun, DU Yang, ZHAO Feng-Jiao, CUI Meng, LIU Zhi-Qiang. Electrospray Ionization Tandem Mass Spectrometry on the Interaction of Baicalin and Glutathione[J]. Chinese Journal of Applied Chemistry, 2021, 38(3): 331-342.

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Electrospray Ionization Tandem Mass Spectrometry on the Interaction of Baicalin and Glutathione

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