Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp.B21

doi:10.11944/j.issn.1000-0518.2015.10.150076

Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (10): 1196-1200.DOI: 10.11944/j.issn.1000-0518.2015.10.150076

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Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp.B21

LUO Youping,DENG Pengfei(),NIU Yanyan,ZHENG Caijuan,YAN Xiangyang,CHEN Jun

Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China

Received:2015-03-03 Accepted:2015-06-01 Published:2015-10-10 Online:2015-10-10
Contact: DENG Pengfei
Supported by:
Supported by the Natural Science Foundation of Hainan Province(No.214023)

Abstract

Abstract:

The secondary metabolites of the medicinal mangrove endophytic fungus Penicillium sp.B21 were investigated. Five compounds were firstly isolated from the ethyl acetate extract of the fermentation mixture using silica gel column chromatography, preparative thin-layer chromatography and recrystallization. The structures of these compounds were identified as janthinone(1), emodin(2), citreorosein(3), decarboxydihydrocitrinone(4) and sclerotinin B(5), by comprehensive spectroscopic characterizations, X-ray single crystal diffraction as well as comparison with reported data. The single crystal structure of compound 5 was firstly obtained.

Key words: mangrove endophytic fungus, Penicillium sp. B21, secondary metabolites, isolation and elucidation, X-ray single crystal diffraction

LUO Youping,DENG Pengfei,NIU Yanyan,ZHENG Caijuan,YAN Xiangyang,CHEN Jun. Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp.B21[J]. Chinese Journal of Applied Chemistry, 2015, 32(10): 1196-1200.

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Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp.B21

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[1]	LI Shangde1*, WEI Meiyan1, LI Zhihua1, SHE Zhigang2, LIN Yongcheng2. Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp. (ZZF29#) [J]. Chinese Journal of Applied Chemistry, 2012, 29(06): 727-729.
[2]	HUANG Zhong-Jing1,2, YANG Run-Yun2,3, GUO Zhi-Yong2,4, SHE Zhi-Gang2, LIN Yong-Cheng2*. New Anthraquinone Derivative Produced by Cultivation of Mangrove Endophytic Fungus Fusarium sp. ZZF60 from the South China Sea [J]. Chinese Journal of Applied Chemistry, 2010, 27(04): 394-397.