Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography

doi:10.3724/SP.J.1095.2014.30299

Chinese Journal of Applied Chemistry

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Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography

FAN Yanqun^1,2, XU Jianzhong², XU Xun^1,2, LIU Yijie³, XU Chen^2*

(1.School of Life Science,Xiamen University,Xiamen 361102,China;
2.Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,China;
3.Fujian Medical University,Fuzhou 350108,China)

Received:2013-06-17 Revised:2013-08-19 Published:2014-04-10 Online:2014-04-10
Contact: Chen Xu

Abstract

Abstract: An analytical method was established for the simultaneous determination of glucuronic acid and glucuronolactone using ion-exclusion chromatography. The separation was performed on a Boston HC-B75 H+ column at 25 ℃ by HPLC equipped with an ultraviolet detector at the wavelength of 220 nm. The mobile phase is 5 mmol/L sulfuric acid with a flow rate of 0.6 mL/min. The quantification was performed by an external standard. The linear ranges for glucuronic acid and glucuronolactone are 10～1200 mg/L and 5～600 mg/L(r＞0.9999), respectively. The detection and quantification limits for the two compounds are 0.1 mg/L and 0.3 mg/L, respectively. The standard deviations for glucuronic acid and glucuronolactone are 1.00% and 0.35%, respectively. The recovery ratios for glucuronic acid and glucuronolactone are 99.69% and 98.21%, respectively. This method is convenient, rapid, sensitive and suitable for the simultaneous determination of glucuronic acid and glucuronolactone. Temperature, pH and solvent affected the stability of glucuronic acid.

Key words: ion-exclusion chromatography, glucuronic acid, glucuronolactone, stability

CLC Number:

O657.7

FAN Yanqun1,2, XU Jianzhong2, XU Xun1,2, LIU Yijie3, XU Chen2*. Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30299.

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Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography

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