A GLYCEROLPROPYL-DIETHYLENESILYL BONDED PHASE FOR AMINO ACID SEPARATION AND DETERMINATION

Chinese Journal of Applied Chemistry ›› 1992, Vol. 0 ›› Issue (2): 106-110.

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A GLYCEROLPROPYL-DIETHYLENESILYL BONDED PHASE FOR AMINO ACID SEPARATION AND DETERMINATION

Da Shilu, Liu Wuping

Department of Chemistry, Wuhan University, Wuhan 430072

Received:1991-02-25 Revised:1991-08-22 Published:1992-04-10 Online:1992-04-10

Abstract

Abstract: Separation and determination of amino acids were carried out by means of a glycerolpropyl-diethylenesilyl bonded phase (GDSB) combined with a UV-detector at 254nm. An acetate buffer solution (pH 5.75) containing 2.5?10^-4 mol/L of copper ion and 2.5?10^-4 mol/L of hexyl sulfonate was used as a mobile phase. The effects of the mobile phase composition on capacity factor and detection sensitivity were examined. The results the suifability of GDSB system as a stationary phase for analysis of amino acids showed by HPLC.

Key words: glycerolpropyl-diethylenesilyl bonded phase, Amino acid, chromatographic analysis

Da Shilu, Liu Wuping. A GLYCEROLPROPYL-DIETHYLENESILYL BONDED PHASE FOR AMINO ACID SEPARATION AND DETERMINATION[J]. Chinese Journal of Applied Chemistry, 1992, 0(2): 106-110.

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A GLYCEROLPROPYL-DIETHYLENESILYL BONDED PHASE FOR AMINO ACID SEPARATION AND DETERMINATION

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