CATALASE ENZYME ELECTRODE AND ITS PROPERTY

Chinese Journal of Applied Chemistry ›› 1992, Vol. 0 ›› Issue (1): 89-91.

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CATALASE ENZYME ELECTRODE AND ITS PROPERTY

Lu Zhongqing, Zhou Zuxin, Cao Dajun, Zhang Zongrang

Department of Chemistry, Shanghai Teachers' University, Shanghai 200234

Received:1990-11-27 Revised:1991-04-05 Published:1992-02-10 Online:1992-02-10

Abstract

Abstract: A catalase membrane immobilized on a nylon net was prepared, using freshly synthesized triethylonium tetrafluoroborate as immobilizer. The membrane had an activity of 80unit/cm²,while that immobilized by dimethyl sulfate showed an activity only of 35, 6unit/cm² .The catalase electrode showed linear response within 5, 0×10^-6～5,0×10^-4mol·L^-1,with detection lirit of 4,0×10^-6mol·L^-1 and 95,2% recovery.Its service life is more than 500 times. SO₄^2-, Cl^-, NO₃^-,NH₄⁺,Ac^-,glycine,glueose etc. do not interfere with the detection.

Key words: catalase, enzyme electorde, immobilization, hydrogenperoxide determination

Lu Zhongqing, Zhou Zuxin, Cao Dajun, Zhang Zongrang. CATALASE ENZYME ELECTRODE AND ITS PROPERTY[J]. Chinese Journal of Applied Chemistry, 1992, 0(1): 89-91.

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CATALASE ENZYME ELECTRODE AND ITS PROPERTY

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