Abstract:

This paper reports the preparation of an acetylcholinesterase(AChE)/Nafion/Prussian blue(PB)/glass carbon electrode(GCE) and the electrochemical detection of pesticides such as carbaryl and trichlorfon. Atomic force microscopy and electrochemical technique were used to investigate the construction of the modified electrode and its effects on pesticide detection. Those results indicated that AChE dispersed uniformly in Nafion/PB/GCE. The AChE/Nafion/PB/GCE constructed under optimal conditions showed a good linear relationship between the peak current and the concentration of pesticide in the range of 0.01~0.5 μmol/L & 2.0~10.0 μmol/L for carbaryl and 0.02~1.0 μmol/L & 2.0~8.0 μmol/L for trichlorfon, respectively. The limit of detection is 5.0 nmol/L for carbaryl and 10.0 nmol/L for trichlorfon, respectively. Furthermore the modified electrode was applied in the detection of analogous practical samples. It was found that the as-prepared sensor had high detection sensitivity, good reproducibility and anti-interference.

Key words: Carbaryl, Trichlorfon, Prussian blue, Nafion, Acetylcholinesterase, Electrochemistry