Abstract:

An ultrasensitive resonance rayleigh scattering(RRS) method for the determination of trace octreotide was developed. The determination of octreotide in an octreotidemethyl red system via RRS was studied comprehensively. In a pH=3.78 B-R buffer solution, the RRS intensity is enhanced obviously in the range of 280~370 nm as octreotide associates with methyl red. The strongest ΔIRRS is at 310 nm. The RRS intensitiy of octreotide is proportional to the concentration of octreotide over the range of 0~0.54 mg/L, the detection limit for the octreotide(3σ) is 3.6 μg/L under optimum conditions. The method was applied to the determination of octreotide in synthetic samples with the recovery of 98.3%~100.7%. Measurements of the commercial injection samples also gave satisfactory results which were in good agreement with those obtained using high performance liquid chromatography(HPLC) method.

Key words: Resonance Rayleigh Scattering, Octreotide, Methyl red