Nd3+ Sensitized Fluorescent Nanoprobes for Vascular Imaging in the Second Near Infrared Window

doi:10.19894/j.issn.1000-0518.210580

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (4): 685-693.DOI: 10.19894/j.issn.1000-0518.210580

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Nd³⁺ Sensitized Fluorescent Nanoprobes for Vascular Imaging in the Second Near Infrared Window

Song-Tao ZHANG¹^,², Ying-Hui WANG¹(), Hong-Jie ZHANG¹^,²()

^1.State Key Laboratory of Rare Earth Resource Utilization，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022，China
^2.University of Chinese Academy of Sciences，Beijing 100049，China
^3.Department of Chemistry，Tsinghua University，Beijing 100084，China

Received:2021-12-28 Accepted:2022-03-08 Published:2022-04-01 Online:2022-04-19
Contact: Ying-Hui WANG,Hong-Jie ZHANG
Supported by:
National Natural Science Foundation of China(52022094);the Youth Innovation Promotion Association of Chinese Academy of Sciences(2019232)

Abstract

Abstract:

In recent years， in comparison to the light in the visible range （400~700 nm） and first near-infrared window （NIR-Ⅰ， 700~1000 nm）， the light in the second near-infrared window （NIR-Ⅱ， 1000~1700 nm） possess the merits of the lowest optical scattering and absorption in the bio-tissue. So NIR-Ⅱ fluorescence imaging with deeper tissue penetration depths， high spatiotemporal resolution and signal to noise ratio （SNR） plays an important role in the fields of angiography， lymphangiography and so on. Herein， Nd³⁺sensitized fluorescent nanoprobe （U/DNPs， NaGdF₄∶Yb（18% mole fraction），Ho（2%），Ce（30%）@NaGdF₄，Nd（60%）@NaGdF₄，Nd（10%）） with core-shell-shell is synthesized through high-temperature coprecipitation and epitaxial growth method. Under the irradiation of 808 nm， the NIR-Ⅱ fluorescence at 1056 nm and 1332 nm are obtained via down-conversion process for further vascular imaging. Furthermore， it can emit red upconversion fluorescence with high red to green （R/G） ratio （I₆₄₇/I₅₄₀= 22.6）， possessing greatly potential application for photodynamic therapy. After successful modification of polyvinyl pyrrolidone （PVP） via ligand-exchange strategy， the U/DNPs-PVP nanomaterials possess excellent solubility in water and good biocompatibility， and can achieve the NIR-Ⅱ fluorescence vessels imaging with spatial resolution of 336.3 μm in vivo.

Key words: Rare earth ions, Second near-infrared window, Vascular imaging, Fluorescence imaging

CLC Number:

O614.33

Song-Tao ZHANG, Ying-Hui WANG, Hong-Jie ZHANG. Nd³⁺ Sensitized Fluorescent Nanoprobes for Vascular Imaging in the Second Near Infrared Window[J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 685-693.

Figures/Tables 7

Fig.1 TEM images and size distributions of nanoparticles with different structures and doped concentrations

Fig.2 XRD patterns of nanoparticles with different structures and doped concentrations

Fig.3 The up-conversion emission （A） and down-conversion （B） spectra of Core@NaGdF4，Nd（30%， 60% and 90%） nanomaterials with core-shell structure

Fig.4 The down-conversion spectra of Core@Shell and Core@Shell@NaGdF4，Nd （10% and 20%） nano materials with core-shell-shell structure

Fig.5 （A） FT-IR spectra，（B） hydrodynamic diameter and （C） Zeta potential of U/DNPs-PVP nanomaterials

Fig.6 The cell viability of U/DNPs-PVP with different mass concentration （25， 50， 100， 200 and 400 μg/mL）

Fig.7 （A） The NIR-Ⅱ imaging of U/DNPs-PVP in vivo. （B） and （C） The cross-sectional signal intensity profiles of vessels. Scale bars： 5 mm

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Nd³⁺ Sensitized Fluorescent Nanoprobes for Vascular Imaging in the Second Near Infrared Window

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