Separation and Determination of Norfloxacin in Real Water Samples Using Binary Small Molecule Alcohol-Salt Aqueous Two-Phase  System Coupled with High Performance Liquid Chromatography

doi:10.19894/j.issn.1000-0518.200331

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (7): 847-856.DOI: 10.19894/j.issn.1000-0518.200331

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Separation and Determination of Norfloxacin in Real Water Samples Using Binary Small Molecule Alcohol-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

GUO Dong-Gang^1*, NI Liang^1,2, WANG Liang¹

¹Key Laboratory of Preparation and Application of Environmental Friendly Materials, Jilin Normal University, Ministry of Education, Changchun 130103, China
²School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China

Received:2020-11-04 Accepted:2021-01-20 Published:2021-07-01 Online:2021-09-01
About author:National Natural Science Foundation of China (No.21407058)

Abstract

Abstract: A binary aqueous two-phase system (ATPS) based on ethanol-1-propanol-NaH₂PO₄ system was constructed and applied for the separation and determination of norfloxacin (NOR) in real water samples. The influence factors on partition behaviors of NOR were discussed, including the types and the concentration of phase salts, the volume ratio of alcohols, the pH value and extraction temperature. The response surface methodology was used to determine the best experimental conditions for multi-factor experiments. The study shows that when the concentration of NaH₂PO₄ is 0.200 g/mL, the volume of ethanol is 2 mL (the volume ratio of alcohols is 1∶1) , pH value is 5, and the extraction temperature is 30 ℃, the extraction efficiency of NOR reaches 94.34%. Compared with the unit aqueous two-phase system, the extraction efficiency of the target compound by the multi-component aqueous two-phase system constructed in this paper is obviously improved.

Key words: Multi-component mixed aqueous two-phase system, Small molecule alcohol, Extraction/detection, Norfloxacin

CLC Number:

O658.2

GUO Dong-Gang, NI Liang, WANG Liang. Separation and Determination of Norfloxacin in Real Water Samples Using Binary Small Molecule Alcohol-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography[J]. Chinese Journal of Applied Chemistry, 2021, 38(7): 847-856.

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Separation and Determination of Norfloxacin in Real Water Samples Using Binary Small Molecule Alcohol-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

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