Identification and Content Determination of Active Constituents of Fig Leaves

doi:10.11944/j.issn.1000-0518.2018.11.180051

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (11): 1391-1398.DOI: 10.11944/j.issn.1000-0518.2018.11.180051

• Full Papers • Previous Articles

Identification and Content Determination of Active Constituents of Fig Leaves

ZHAO Mohan^a,LIU Zhiqiang^b,GENG Aifang^a^*(),XIE Wenbing^b^*()

^aSchool of Chemical and Environmental Engineering,Changchun University of Science and Technology,Changchun 130022,China
^bNational Analytical Research Center of Electrochemistry and Spectroscopy,Changchun Institute of AppLied,Chemistry Chinese Academy of Sciences,Changchun 130022,China

Received:2018-02-26 Accepted:2018-06-05 Published:2018-10-31 Online:2018-10-31
Contact: GENG Aifang,XIE Wenbing
Supported by:
Supported by the National Natural Science Foundation of China(No.81473537)

Abstract

Abstract:

In recent years, studies on the extraction and analysis of the components contained in fig leaves have focused on the analysis of the same type or the whole group of single compounds, and there are few qualitative and quantitative studies on single effective substances in various types. In this study, solvent extraction-ultrasonic-assisted extraction was used to extract the active ingredients of fig leaves, and the active ingredients were identified by gas chromatography-mass spectrometry and ultrahigh performance liquid chromatography-ion trap-mass spectrometry. The results show that the main components of fig leaves are chlorogenic acid, rutin, psoralen and bergapten. The single factor experiments were used to determine the best extraction process for the four components. A quantitative method for the determination of chlorogenic acid, rutin, psoralen and bergapten was established by high performance liquid chromatography. The linear relationship is obtained for chlorogenic acid in the range of 1.84~9.2 μg(R²=0.9991) with recovery rate of 95.2%, rutin in the range of 1.84~9.2 μg(R²=0.9981) with a recovery rate of 96.8%, bergapten in the range of 4~20 μg(R²=0.9998) with a recovery rate of 89.5%, and psoralen in the range of 4~20 μg(R²=0.9963) with a recovery rate of 87.1%, respectively.

Key words: fig leaves, chlorogenic acid, rutin, psoralen, bergapten

ZHAO Mohan,LIU Zhiqiang,GENG Aifang,XIE Wenbing. Identification and Content Determination of Active Constituents of Fig Leaves[J]. Chinese Journal of Applied Chemistry, 2018, 35(11): 1391-1398.

References

[1]	WANG Haiyan,LIU Bingbo,GUO Aixin,et al. Clinical Observation of Fig Leaf Fumigation with Acupuncture in 45 Patients with Hemorrhoids[J]. Chinese J Basic Med Tradit Chinese Med,2013,19(10):1181-1183(in Chinese). 王海燕,刘炳波,郭爱新,等. 无花果叶熏洗联合针灸治疗痔疮45例临床观察[J]. 中国中医基础医学杂志,2013,19(10):1181-1183.
[2]	Mawa W,Husain H,Jantan I.Ficus carica L.(Moraceae):Phytochemistry, Traditional Uses and Biological Activities[J]. Evid-Based Compl Altern Med,2013,2013(2013):1-8.
[3]	HUANG Dandan.Extraction and Hypoglycemic Activity of Flavonoids in Ficus Carica Leaves[D]. Fujian:Fujian Medical University,2015(in Chinese). 黄丹丹. 无花果叶总黄酮的提取及降血糖作用研究[D]. 福建:福建医科大学,2015.
[4]	ZHANG Zejun,SHA Kun.Study on Extraction of Total Flavonoids from Ficus carica Leaves by Ultrasound-assisted Method[J]. Food Res Dev,2011,6(32):14-16(in Chinese). 张泽俊,沙坤. 超声波辅助提取无花果叶中总黄酮工艺的研究[J]. 食品研究与开发,2011,6(32):14-16.
[5]	WANG Zhenbin,LIU Jiayou,MA Haile,et al. Extraction Process Optimization and Ultrasonic Modification of Polysaccharide from Ficus carica L.[J]. Trans Chinese Soc Agric Eng,2014,30(10):262-269(in Chinese). 王振斌,刘加友,马海乐. 无花果多糖提取工艺优化及其超声波改性[J]. 农业工程学报,2014,30(10):262-269.
[6]	ZHANG Junyan,PENG Shanshan,FANG Yuan,et al. Extraction by Ultrasonic Method and Determination of Polysaccharide from Lycium Barbarum Leaves and Ficus caria Leaves[J]. Food Ind,2011,32(12):98-100 (in Chinese). 张俊艳,彭珊珊,方圆,等. 枸杞叶、无花果叶多糖的超声波提取和测定[J]. 食品工业,2011,32(12):98-100.
[7]	DeL Caro A,Piga A. Polyphenol Composition of Peel and Pulp of Two Italian Fresh Fig Fruits Cultivars(Ficus carica L.)[J]. Eur Food Res Technol,2008,226(4):785-719.
[8]	Veberic R,CoLaric M,Stampar F. Phenolic Acids and Flavonoids of Fig Fruit(Ficus carica L.) in the Northern Mediterranean Region[J]. Food Chem,2008,106(1):153-157.
[9]	Dilaram Nijat,Hajiakber Aisa.Determination of Three Coumarins in Ficus carica L. Latex by HPLC Methods[J]. J Xinjiang Med Univ,2016,1(1):52-55(in Chinese). 迪丽阿热姆·尼加提,阿吉艾克拜尔·艾萨. HPLC法测定阿图什无花果叶汁中3种香豆素的含量[J]. 新疆医科大学学报,2016,1(1):52-55.
[10]	Wang T,Jiao J,Gai Q Y,et al. Enhanced and Green Extraction Polyphenols and Furanocoumarins from Fig(Ficus carica L.) Leaves Using Deep Eutectic Solvents[J]. J Pharm Biomed Anal,2017,10(25):339-345.
[11]	Takahashi T,Okiura A,Kohno M.Phenylpropanoid Composition in Fig(Ficus carica L.) Leaves[J]. J Nat Med,2017,71(4):770-775.
[12]	Mahmoudi S,Khali M,Benkhaled K,et al. Phenolic and Flavonoid Contents, Antioxidant and Antimicrobial Activities of Leaf Extracts from Ten Algerian Ficus carica L. Varieties[J]. Asian Pac J Trop Biomed,2016,6(3):239-245.
[13]	PatiL Vikas V,BhangaLe S C,Patil V R. Evaluation of Ant-inflammatory Activity of Ficus carica Linn. Leaves[J]. Indian J Nat Prod Resour,2010,2(2):48-50.
[14]	Shih Y Z,Huang A J,Hou C Y,et al. The Stimulating Effects of Polyphenol and Protein Fractions from Jelly Fig(Ficus awkeotsang Makino) Achenes Against Proliferation of Leukemia Cells[J]. J Food Drug Anal,2017,10(4):854-861.
[15]	ZHANG Heliang.Study on the Preparation of Fig Lesves' Extracts and Their Antibacterial Effect[D]. Shandong:Qilu University Technology,2015(in Chinese). 张合亮. 无花果叶提取物的制备及其抑菌效果研究[D]. 山东:齐鲁工业大学,2015.
[16]	DU Wenjing,LUO Li,LUO Demei,et al. Effect of Ficus carica L Leaf on Osteoporosisof OPG Knockout Mice[J]. China J Tradit Chinese Med Pharm,2015,30(10):3687-3690(in Chinese). 杜文静,罗莉,罗德梅,等. 无花果叶提取物对OPG基因敲除小鼠的抗骨质疏松作用研究[J]. 中华中医药杂志,2015,30(10):3687-3690.
[17]	XU Qiuxia,ZHANG Yin,HUANG Dandan,et al. Effects of Ficus carica Leaves Extracts on Blood Glucose Level and Antioxidative Ability in Diabetic Rats[J]. J Fujian Med Univ,2013,47(3):146-149(in Chinese). 许秋霞,张吟,黄丹丹,等. 无花果叶提取物对糖尿病大鼠血糖及抗氧化能力的影响[J]. 福建医科大学学报,2013,47(03):146-149.
[18]	Takahashi T,Okiura A,Kohno M.Identification of Phenylpropanoid in Fig(Ficus carica L.) Leaves[J]. J Agric Food Chem,2014,62(41):10076-10083.
[19]	LI Jun,SU Fan,CHEN Jipin,et al. Determination of Psoralen and Bergapten in Leaves of Ficua carica L. in Atushi by HPLC[J]. Chinese J Med Guide,2009,11(9):1607-1609(in Chinese). 李军,苏凡,陈吉平,等. HPLC法测定阿图什市无花果叶中补骨脂素及佛手苷内酯含量[J]. 中国医药导刊,2009,11(9):1607-1609.
[20]	ZHANG Zongyuan,LI Maohua,ZHANG Tao.Spectrophotometric Determination of Tannin in Xinjiang Fig Leaves[J]. Chinese J Anal Lab,2009,5(28):188-190(in Chinese). 张纵圆,李茂华,张涛. 分光光度法测定新疆无花果叶中的单宁[J]. 分析实验室,2009,5(28):188-190.
[21]	LIU Yi,CHEN Li.Comparison of Psoralen Contents of Fig Leaves Before and After Processing[J]. Herald Med,2012,31(05):660-662(in Chinese). 刘毅,陈莉. 无花果叶炮制前后补骨脂素含量比较[J]. 医药导报,2012,31(5):660-662.
[22]	CUI Peng,CHEN Zhiran,SHI Leige.Microwave Extraction and Free Redical Scavenging Activity of Total Flavonnids from Ficus carica L. Leaves[J]. Food Res Dev,2018,39(1):41-45(in Chinese). 崔鹏,陈志冉,史雷革. 微波提取无花果叶中的总黄酮及其体外抗氧化活性研究[J]. 食品研究与开发,2018,39(1):41-45.
[23]	Nijat SAWUT,YU Yang,Suleyman Halik.RP-HPLC Determination of Rutin and Psoralen in Leaves of Ficua carica L[J]. Mod Chinese Med,2012,14(10):16-19(in Chinese). 尼加提·沙吾提,鱼洋,苏来曼·哈力克. RP-HPLC同时测定维吾尔药材无花果叶中芦丁和补骨脂素含量[J]. 中国现代中药,2012,14(10):16-19.
[24]	ZHANG Yin,CHEN Yinong,XU Qiuxia,et al. Analysis of Ficua carica Leaves Extraction by GC-MS and UHPLC/DAD[J]. J Fujian Med Univ,2011,45(06):423-426(in Chinese). 张吟,陈一农,许秋霞,等. 气质联用色谱法和超高效液相色谱法检测无花果叶提取物的化学成分[J]. 福建医科大学学报,2011,45(6):423-426.

Identification and Content Determination of Active Constituents of Fig Leaves

RichHTML

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	WAGN Susu, ZHANG Yue, LI Hui, XU Miaomiao. Preparation, Characterization and Recognition Behavior of Quercetin-Rutin Bi-template Molecularly Imprinted Polymers [J]. Chinese Journal of Applied Chemistry, 2015, 32(11): 1290-1298.
[2]	QIAN Hua*, LIU Da-Bin, WANG Liang. New synthetic Method of 4-Methoxy-7H-Furo[3,2-g][1]benzopyran-7-one [J]. Chinese Journal of Applied Chemistry, 2009, 26(09): 1084-1087.
[3]	Wang Jun, Song Yulin, Hu Xuejun, Duan Jinglin. THE COMPLEXES OF RARE EARTH IONS RE(Ⅲ)WITH RUTIN [J]. Chinese Journal of Applied Chemistry, 1989, 0(5): 100-101.