In vivo Immune Response Using Tobacco Mosaic Virus as Hapten Carrier

Chinese Journal of Applied Chemistry

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In vivo Immune Response Using Tobacco Mosaic Virus as Hapten Carrier

Xia ZHAO^a,Limin CHEN^a,Jun HU^a,Xiaolei ZHANG^a,Jittima Amie LUCKANAGUL^b,Yuan LIN^a^*(),Qian WANG^b^*()

^aChangchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
^bUniversity of South Carolina,South Carolina 29208,USA

Published:2015-10-10 Online:2015-10-10
Contact: Yuan LIN,Qian WANG

Abstract

Abstract:

Using a lysine mutant of tobacco mosaic virus(TMV-EPMK) as the hapten carrier, artificial TMV-EPMK/E3 antigens were fabricated by conjugating a small molecular estriol(E3) onto TMV-EPMK through “click” chemistry, and their immune response was evaluated in BALB/c mice. The results demonstrated that TMV-EPMK/E3 antigen containing the hydrophilic linker could enhance the E3 specific immune response with the higher IgG antibody level(titer 21800) by displaying E3 on TMV surface with distinctive topology. This study investigated that immune response was affected by linker property using TMV as the hapten carrier. A hydrophilic linker could elicit a stronger immune response.

Key words: TMV-EPMK, click chemistry, hapten, artificial antigen, immune response

Xia ZHAO, Limin CHEN, Jun HU, Xiaolei ZHANG, Jittima Amie LUCKANAGUL, Yuan LIN, Qian WANG. In vivo Immune Response Using Tobacco Mosaic Virus as Hapten Carrier[J]. Chinese Journal of Applied Chemistry.

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In vivo Immune Response Using Tobacco Mosaic Virus as Hapten Carrier

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