Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen

doi:10.3724/SP.J.1095.2013.20570

Chinese Journal of Applied Chemistry

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Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen

YIN Xiaomei, YU Haitao^*, WANG Xiaolong

(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China)

Received:2012-12-10 Revised:2013-03-15 Published:2013-11-10 Online:2013-11-10
Contact: yu

Abstract

Abstract: FT3C hapten was synthesized via a fast, mild “Click Chemistry”, which contained a “spacer arm” of triazole ring conjugated to carboxyl site of free triiodothyronine(FT3). Immunogen and coating antigen of FT3C were respectively prepared by the coupling reaction between hapten and bovine serum albumin(BSA) or keyhole limpet hemocyanin(KLH), using the N-hydroxysuccinimide active ester method. UV spectrum of the artificial antigen(FT3C-BSA) differed significantly from those of hapten(FT3C) and BSA, the results also showed that FT3C was conjugated to BSA successfully, the coupling ratio of hapten to BSA was 7∶1; the antibody with high titer（6.24×104） and IC50 value of 0.37 mg/L was produced through immunization of Balb/c mice. This study provided a new way to prepare FT3 artificial antigen.

Key words: free triiodothyronine, Click Chemistry, hapten, artificial antigen

CLC Number:

O629.8

YIN Xiaomei, YU Haitao*, WANG Xiaolong . Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2013.20570.

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Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen

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