Abstract:

The bindings of small molecular oxazine750 to d[TG₄T]₄, d[G₄T₄G₄]₂ and c-myc sequences was characterized by means of circular dichroism, thermal denaturation, and so on. Oxazine750 was found to bind preferentially and strongly to parallel G-quadruplex. It can effectively stabilize the parallel G-quadruplex and destabilize the antiparallel G-quadruplex. The melting temperature of d[TG₄T]₄ or c-myc is increased by about 11 ℃ or 40 ℃ by oxazine750, respectively. Under the same condition, oxazine750 decreased the melting temperature of d[G₄T₄G₄]₂ by about 8 ℃.

Key words: G-quadruplex, small molecular, competition dialysis