Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+

doi:10.3724/SP.J.1095.2010.90548

Chinese Journal of Applied Chemistry ›› 2010, Vol. 27 ›› Issue (06): 737-741.DOI: 10.3724/SP.J.1095.2010.90548

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Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+

TAN Ke-Jun*, MA Lu, QIN Ming-Li

(Education Ministry Key Laboratory on Luminescence and Real-Time Analysis,College of
Chemistry and Chemical Engineering,Southwest University,Chongqing 400715)

Received:2009-08-24 Revised:2009-10-16 Published:2010-06-10 Online:2010-06-10

Abstract

Abstract:

In Tris-HCl buffer solution, a resonance light scattering(RLS) detection method for adenosine disodium triphosphate(ATP) was developed based on RLS signal enhancement due to interactions of ATP with cerium ions in an alkalescent aqueous medium. The enhanced RLS intensity was proportional to ATP content in a range of 2.0~24 μmol/L with a limit of determination(LOD,S/N=3σ) of 51.25 nmol/L. The effects of coexisting substances were investigated. The method has been successfully applied to determining the content of ATP in injection samples with a RSD of 2.29%.

Key words: Adenosine disodium triphosphate, cerium ions, resonance light scattering

CLC Number:

O657.3

TAN Ke-Jun*, MA Lu, QIN Ming-Li. Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+[J]. Chinese Journal of Applied Chemistry, 2010, 27(06): 737-741.

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Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+

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