Spectroscopic Study on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin

Chinese Journal of Applied Chemistry ›› 2009, Vol. 26 ›› Issue (08): 918-922.

• Full Papers • Previous Articles Next Articles

Spectroscopic Study on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin

YUAN Dao-Qin, FENG Su-Ling*, CUI Feng-Ling

(College of Chemistry and Environmental Science,Henan Normal University,Xinxiang 453007）

Received:2008-07-23 Revised:2008-10-06 Published:2009-08-10 Online:2009-08-10

Abstract

Abstract:

The interaction between diammonium glycyrrhizinate and bovine serum albumin was studied in physicological solution by spectroscopic methods including fluorescence spectra and UV–visible absorption spectra. Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the conformation change of bovine serum albumin due to the addition of diammonium glycyrrhizinate. It showed that the dynamic quenching exited between diammonium glycyrrhizinate and bovine serum albumin. Based on the equation of Stern-Volmer, the quenching constant was obtained. According to Förster theory of non-radiation energy transfer, the distance between bovine serum albumin and diammonium glycyrrhizinate was determined.

Key words: diammonium glycyrrhizinate, bovine serum albumin, fluorescence spectra, absorption spectra, synchronous fluorescence, three-dimensional fluorescence spectra

CLC Number:

0644.17

YUAN Dao-Qin, FENG Su-Ling*, CUI Feng-Ling. Spectroscopic Study on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2009, 26(08): 918-922.

[1]	Xing-Mei HU, Jin-Hong ZHANG, Yu-Lin MO, Hai-Bin LIN. Designing Dual-output Digital Logic Circuits Based on the Combination of Bovine Serum Albumin and Schiff Base [J]. Chinese Journal of Applied Chemistry, 2023, 40(6): 904-915.
[2]	XIE Xingqin. Interaction Mechanism Between Thiourea Aryl Iridium Anticancer Complex and Bovine Serum Albumin [J]. Chinese Journal of Applied Chemistry, 2020, 37(5): 604-610.
[3]	LIU Wei, YANG Shuilan, SONG Pan, YANG Tianlin. Synthesis and Characterization of a Phosphorus-containing Tripod Ligand and Its Europium Comphex [J]. Chinese Journal of Applied Chemistry, 2015, 32(7): 777-787.
[4]	CHEN Cailian, ZHU Qingren, SUN Dengming. Studies on the Interaction of Taxifolin and Bovine Serum Albumin by Spectroscopic and Voltammetric Methods [J]. Chinese Journal of Applied Chemistry, 2014, 31(05): 617-623.
[5]	GENG Shaoguang, JIANG Xiaoying, CUI Fengling*, SHI Lei. Determination of Protein in Biological Samples Using a Thiosemicarbazone Derivative as a Fluorescence Probe [J]. Chinese Journal of Applied Chemistry, 2013, 30(05): 555-559.
[6]	LIU Fan*, ZHANG Yuanqin, ZHANG Zhijin. Interaction of 1,4,7,10-Tetraazacyclododecane-Co(Ⅱ) with Bovine Serum Albumin [J]. Chinese Journal of Applied Chemistry, 2013, 30(02): 232-238.
[7]	WU Fei, ZHU Jin, TAN Kejun*. Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application [J]. Chinese Journal of Applied Chemistry, 2012, 29(08): 969-973.
[8]	FENG Xiaoqiang*, LI Xiaofang, YANG Sheng. Preparation, Antibacterial Activity of Thiourea-chitosan-Co(II) Complex and Interaction with Bovine Serium Albumin [J]. Chinese Journal of Applied Chemistry, 2011, 28(09): 1012-1016.
[9]	XING Weiwei, CUI Fengling*, MAO Runze, CHENG Shan, HUO Ruina. Synchronous Fluorescence Spectrometric Determination of Proteins with Resveratrol as Probe [J]. Chinese Journal of Applied Chemistry, 2011, 28(07): 849-854.
[10]	NING Aimin*, DANG Yuli, ZHAO Shiju, WAN Xinsheng, ZHENG Xianfu. Study on the Interaction of Protein with Quaternary-ammonium Gemini Surfactants by Electromotive Force Method [J]. Chinese Journal of Applied Chemistry, 2011, 28(07): 826-830.
[11]	ZHANG Zhao-Hong1*, HAN Wen-Ming1, WANG Dong-Mei2, XU Dan-Ping1, DENG Ying-Qiao1, CHEN Zhong-Lin1, GAO Wei1. Study on the Damage of Bovine Serum Albumin under Visible Light in the Presence of Heat-Treated Titanium Dioxide [J]. Chinese Journal of Applied Chemistry, 2010, 27(05): 594-599.
[12]	LIANG Jing, ZHANG Xin-Ying, FENG Su-Ling*, FAN Xue-Sen. Fluorescence Spectra of the Interaction Between Pyrano [4,3-b] Pyran Derivative and Bovine Serum Albumin [J]. Chinese Journal of Applied Chemistry, 2010, 27(05): 600-605.
[13]	ZHANG Gen-Cheng*, XU Jie-Yan. Studies on the interaction between chromium (VI) and bovine serum albumin [J]. Chinese Journal of Applied Chemistry, 2010, 27(02): 191-196.
[14]	. Studied on the determination of proteins in biology samples by synchronous fluorescence technique with 2-Hydroxy-5-nitrobenzoic acid as a probe [J]. Chinese Journal of Applied Chemistry, 2009, 26(6): 730-733.
[15]	ZHANG Jian, QIU Yu, YU Dao-Yong*. Critical Micelle Concentration Determination of Sodium Dodecyl Benzene Sulfonate by Synchronous Fluorescence Spectrometry [J]. Chinese Journal of Applied Chemistry, 2009, 26(12): 1480-1483.

Spectroscopic Study on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments