Chinese Journal of Applied Chemistry

• Full Papers • Previous Articles     Next Articles

Synthesis of Fluorescein Isothiocyanate-labeled Vancomycin and Its Bacterial Detection by Fluorescence

XU Rong1, RUAN Ling'ao2, GE Mei3, ZHANG Yixuan1*   

  1. (1.Shenyang Pharmaceutical University,Shenyang 110072,China;
    2.Shanghai Jiaotong University,Shanghai 200240,China;
    3.Shanghai Laiyi Biological Drug Development Center,Shanghai 200240,China)
  • Received:2013-03-07 Revised:2013-04-22 Published:2014-02-10 Online:2014-02-10
  • Contact: Zhang

Abstract: Fluorescein isothiocyanate(FITC)-labeled vancomycin(FITC-Vanco, FV) was synthesized. After purified by preparative HPLC, the structure of the target compound was indentified by MS. Its combination amount toward bacteria with different concentration was evaluated by the fluorescence intensity of the bacterial suspension. FV synthesized in this study shows specific binding with different bacteria. It shows promising application value in studying bacterial resistance mechanism and bacterial susceptibility to vancomycin.

CLC Number: