Abstract:

The effects of pH value and eluent of phosphate buffer solution on the regeneration of immobilized metal affinity capture arrays-Cu(IMAC-Cu) proteinchip was studied by scanning electron microscope(SEM) and SELDI-TOF-MS proteinchip system. The results show that the adsorbed proteins can be easily removed. However, the negative influence on chemical structure of the proteinchip is getting worse with decreasing the pH value of the phosphate buffer solution. Adding eluent of EDTA or Gly in the phosphate buffer solution can obviously improve the protein desorption. The best regeneration results of IMAC-Cu proteinchip by phosphate solution are gained at pH=6.5 with using 0.02 mol/L EDTA as eluent for 72 h.

Key words: proteinchip, phosphate buffer solution, regeneration