Application Research Progress of Visual Technique in Biomolecular Analysis

doi:10.19894/j.issn.1000-0518.230235

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (1): 21-38.DOI: 10.19894/j.issn.1000-0518.230235

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Application Research Progress of Visual Technique in Biomolecular Analysis

Kai-Xuan LI¹, Jin-Rong CHEN¹, Qian-Qian ZOU², Peng-Fei SHI¹, Li-Shang LIU¹(), Shu-Sheng ZHANG¹()

^1.Medical School，Linyi University，Linyi 276000，China
^2.Clinical Laboratory，Linyi Hospital of Traditional Chinese Medicine，Linyi 276000，China

Received:2023-08-07 Accepted:2023-11-10 Published:2024-01-01 Online:2024-01-30
Contact: Li-Shang LIU,Shu-Sheng ZHANG
About author:Email: shushzhang@126.com
Email: liulishanghao@126.com
Supported by:
the National Natural Science Foundation of China(21675076);the National College Students' Innovation and Entrepreneurship Training Program(202210452011)

Abstract

Abstract:

Visual detection technology is that generates optical signals in the visible region through the specific interaction between the targets and micro-nanoparticles or other materials， and then realizes the quantification of targets with the assistance of the naked eye or image processing technology. The application of visual detection technology in the field of bioanalysis can effectively reduce the detection cost， improve the low efficiency of biological detection， simplify the complicated operation steps， and solve the problems such as the need to use professional instruments. This work introduces the latest research progress of visual detection technology in the field of bioanalysis in recent 15 years. According to the detection principle， it can be classified into particle counting method， colorimetry method， liquid crystal sensing method， range signal method， and lateral flow test strip method. Finally， the advantages and challenges of visual detection technology are analyzed， and the future application prospects of visual detection technology in the field of bioanalysis are prospected.

Key words: Visualization, Biomolecular analysis, Digital counting, Distance signal, Colorimetric analysis

CLC Number:

O625

Kai-Xuan LI, Jin-Rong CHEN, Qian-Qian ZOU, Peng-Fei SHI, Li-Shang LIU, Shu-Sheng ZHANG. Application Research Progress of Visual Technique in Biomolecular Analysis[J]. Chinese Journal of Applied Chemistry, 2024, 41(1): 21-38.

Figures/Tables 23

Fig.1 （A） Schematic of GNP probe-assisted SEM-counting chip［21］；（B） The phage-gold nanobiological material was used as a probe for gross enumeration of target miRNA［22］；（C） Macroscopic counting of target viruses using phage-gold nanoparticles as probes［23］

Fig.2 Schematic of platinum nanoparticle based microbubbling assay［15］

Fig.3 Schematic of the CuP-enhanced naked eye DNA detection method［24］

Fig.4 Liquid crystal-based detection of DNA hybridization using surface immobilized single-stranded DNA［27］

Fig.5 Principles for detection of DNA by using LC （Red probes represent DNA whereas blue probes represent PNA）［32］

Fig.6 The polarized images of the LC-based aptasensor in the presence of the various concentrations of OTA［33］

Fig.7 （A） Naked-eye semiquantitative （NEQ） assay for visual quantification of H2O2. （B） Principle of the proposed NEQ-ELISA for visual quantification of proteins［38］

Fig.8 Schematic of high sensitive colorimetric detection of DNA by using gold nanoparticles and hybridization chain reaction amplification［43］

Fig.9 Schematic diagram of colorimetric gene detection based on CRISPR/Cas［48］

Fig.10 Schematic of the DNA molecular beacon （MB） detection method using the visual scanning strategy［50］

Fig.11 Schematic of pH-resolved colorimetric biosensor for simultaneous multiple targets detection［51］

Fig.12 Schematic diagram of targeted HIV visual detection based on a multiplex amplification nanofiber sensing platform［11］

Fig.13 Schematic diagram of visualization and modular detection of pathogen nucleic acids［52］

Fig.14 Schematic representation of the biomineralization-assisted amplification （BMA） strategy［53］

Fig.15 Schematic diagram of a multistage capacity bar graph chip for DNA visualization and quantification［55］

Fig.16 Au@Pt schematic diagram of nanoparticle-encapsulated target-responsive hydrogel and volumetric bar graph chip readout quantitative point-of-care detection method［56］

Fig.17 Schematic diagram of a microfluidic paper-based Analysis Device （μPAD）［57］

Fig.18 Schematic diagram of an integrated paper-based analysis device for sugar-containing hydrogels based on microfluidic range readout signals［58］

Fig.19 Schematic diagram of a distance-based integrated origami analysis device［59］

Fig.20 Schematic diagram of LAMP amplicon visualization using lateral flow dipstick［60］

Fig.21 Simultaneous detection of miRNA 21 and let-7a using rolling circle amplification combined with lateral flow band specificity［61］

Fig.22 Schematic POCT of Staphylococcus aureus 16S-rRNA based on multiple biotin-labeled DNA probes， side-stream， and smartphone［62］

Fig.23 Schematic diagram of a CRISPR Cas12-based SARS-CoV-2 assay［63］

References 63

1	ZHANG M， YE J， HE J S， et al. Visual detection for nucleic acid-based techniques as potential on-site detection methods. a review［J］. Anal Chim Acta，2020， 1099： 1-15.
2	KAVITA V. DNA biosensors-a review［J］. J Bioeng Biomed Sci， 2017， 7（2）. DOI： 10.4172/2155-9538.1000222.
3	ZHANG L， DING B， CHEN Q， et al. Point-of-care-testing of nucleic acids by microfluidics［J］. TrAC Trends Anal Chem，2017， 94： 106-116.
4	FARKA Z， MICKERT M J， PASTUCHA M， et al. Advances in optical single-molecule detection： en route to supersensitive bioaffinity assays［J］. Angew Chem Int Ed，2020， 59（27）： 10746-10773.
5	MAURER J J. Rapid detection and limitations of molecular techniques［J］. Annu Rev Food Sci Technol，2011， 2： 259-279.
6	DUTTA R， MAKHAIK S， ZHAO P， et al. Colorimetric cotton swab for viral protease detection［J］. Anal Chem，2022， 94（37）： 12699-12705.
7	ZHANG Z， MA P， AHMED R， et al. Advanced point-of-care testing technologies for human acute respiratory virus detection［J］. Adv Mater，2021， DOI： 10.1002/adma.202103646： e2103646.
8	HWANG C， PARK N， KIM E S， et al. Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 （COVID-19）［J］. Biosens Bioelectron，2021， 185： 113177.
9	YANG Z， PENG X， YANG P， et al. A Janus 3D DNA nanomachine for simultaneous and sensitive fluorescence detection and imaging of dual microRNAs in cancer cells［J］. Chem Sci，2020， 11（32）： 8482-8488.
10	WANG R， CHEN R， QIAN C， et al. Ultrafast visual nucleic acid detection with CRISPR/Cas12a and rapid PCR in single capillary［J］. Sens Actuators B： Chem，2021， 326： 128618.
11	LONG Y， ZHOU C， WANG C， et al. Ultrasensitive visual detection of HIV DNA biomarkers via a multi-amplification nanoplatform［J］. Sci Rep，2016， 6： 23949.
12	CHEN Y， CHENG N， XU Y， et al. Point-of-care and visual detection of P.aeruginosa and its toxin genes by multiple LAMP and lateral flow nucleic acid biosensor［J］. Biosens Bioelectron，2016， 81： 317-323.
13	VIETZ C， SCHUTTE M L， WEI Q， et al. Benchmarking smartphone fluorescence-based microscopy with DNA origami nanobeads： reducing the gap toward single-molecule sensitivity［J］. ACS Omega，2019， 4（1）： 637-642.
14	DING X， MAUK M G， YIN K， et al. Interfacing pathogen detection with smartphones for point-of-care applications［J］. Anal Chem，2019， 91（1）： 655-672.
15	CHEN H， LI Z， ZHANG L， et al. Quantitation of femtomolar-level protein biomarkers using a simple microbubbling digital assay and bright-field smartphone imaging［J］. Angew Chem Int Ed，2019， 58（39）： 13922-13928.
16	ABDOLHOSSEINI M， ZANDSALIMI F， MOGHADDAM F S， et al. A review on colorimetric assays for DNA virus detection［J］. J Virol Methods，2022， 301： 114461.
17	SARGAZI M， LINFORD M R， KAYKHAII M. Liquid crystals in analytical chemistry： a review［J］. Crit Rev Anal Chem，2019， 49（3）： 243-255.
18	TIAN T， LI J， SONG Y， et al. Distance-based microfluidic quantitative detection methods for point-of-care testing［J］. Lab Chip，2016， 16（7）： 1139-1151.
19	ZHENG C， WANG K， ZHENG W， et al. Rapid developments in lateral flow immunoassay for nucleic acid detection［J］. Analyst，2021， 146（5）： 1514-1528.
20	LIU X， SUN Y， LIN X， et al. Digital duplex homogeneous immunoassay by counting immunocomplex labeled with quantum dots［J］. Anal Chem，2021， 93（6）： 3089-3095.
21	ZHOU X， YANG C T， XU Q， et al. Gold nanoparticle probe-assisted antigen-counting chip using SEM［J］. ACS Appl Mater Interfaces，2019， 11（7）： 6769-6776.
22	ZHOU X， CAO P， ZHU Y， et al. Phage-mediated counting by the naked eye of miRNA molecules at attomolar concentrations in a Petri dish［J］. Nat Mater，2015， 14（10）： 1058-1064.
23	XU H， SHEN J， YANG C T， et al. Naked-eye counting of pathogenic viruses by phage-gold nanobiomaterials as probes［J］. Mater Today Adv，2021， 10： 100122.
24	KIM J H， PARK J E， LIN M， et al. Sensitive， quantitative naked-eye biodetection with polyhedral Cu nanoshells［J］. Adv Mater，2017， 29（37）： 1702945.
25	MCUMBER A C， NOONAN P S， SCHWARTZ D K. Surfactant-DNA interactions at the liquid crystal-aqueous interface［J］. Soft Matter，2012， 8（16）： 4335-4342.
26	LIU Y， YU J. Oriented immobilization of proteins on solid supports for use in biosensors and biochips： a review［J］. Microchim Acta，2015， 183（1）： 1-19.
27	SHEN J， HE F， CHEN L， et al. Liquid crystal-based detection of DNA hybridization using surface immobilized single-stranded DNA［J］. Microchim Acta，2017， 184（9）： 3137-3144.
28	HU Q Z， JANG C H. Liquid crystal-based sensors for the detection of heavy metals using surface-immobilized urease［J］. Colloids Surfaces B，2011， 88（2）： 622-626.
29	YANG， KUN L， LIU， et al. Applications of metal ions and liquid crystals for multiplex detection of DNA［J］. J Colloid Interface Sci，2015， 439： 149-153.
30	TAN H， YANG S， SHEN G， et al. Signal-enhanced liquid-crystal DNA biosensors based on enzymatic metal deposition［J］. Angew Chem， 2010， 46（122）： 8790-8793.
31	YANG S， WU C， TAN H， et al. Label-free liquid crystal biosensor based on specific oligonucleotide probes for heavy metal ions［J］. Anal Chem，2013， 85（1）： 14-18.
32	LIU Y， YANG K L. Applications of metal ions and liquid crystals for multiplex detection of DNA［J］. J Colloid Interface Sci，2015， 439： 149-153.
33	KHOSHBIN Z， ABNOUS K， TAGHDISI S M， et al. A novel liquid crystal-based aptasensor for ultra-low detection of ochratoxin a using a pi-shaped DNA structure： promising for future on-site detection test strips［J］. Biosens Bioelectron，2021， 191： 113457.
34	WANG H， WU T， LI M， et al. Recent advances in nanomaterials for colorimetric cancer detection［J］. J Mater Chem B，2021， 9（4）： 921-938.
35	SUN J， LU Y， HE L， et al. Colorimetric sensor array based on gold nanoparticles： design principles and recent advances［J］. TrAC-Trends Anal Chem，2020， 122： 115754.
36	WOISKI T D， DE CASTRO PONCIO L， DE MOURA J， et al. Anti-hMC2RL1 functionalized gold nanoparticles for adrenocortical tumor cells targeting and imaging［J］. J Biomed Nanotechnol，2017， 13（1）： 68-76.
37	YANG J， NIAN F， GUO Z， et al. Effect of gold nanoparticles with different diameters on rat neural stem and progenitor cells［J］. Nanosci Nanotechnol Lett，2017， 9（10）： 1491-1496.
38	MA X， CHEN Z， KANNAN P， et al. Gold nanorods as colorful chromogenic substrates for semiquantitative detection of nucleic acids， proteins， and small molecules with the naked eye［J］. Anal Chem， 2016， 88（6）： 3227-323.
39	GILJOHANN D A， SEFEROS D S， DANIEL W L， et al. Gold nanoparticles for biology and medicine［J］. Angew Chem Int Ed，2010， 49（19）： 3280-3294.
40	ZHOU W， GAO X， LIU D， et al. Gold nanoparticles for in vitro diagnostics［J］. Chem Rev，2015， 115（19）： 10575-10636.
41	ELGHANIAN R， STORHOFF J J， MUCIC R C， et al. Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles［J］ Science，1997， 277（5329）： 1078-1081.
42	WILSON R. The use of gold nanoparticles in diagnostics and detection［J］. Chem Soc Rev，2008， 37（9）： 2028-2045.
43	LIU P， YANG X， SUN S， et al. Enzyme-free colorimetric detection of DNA by using gold nanoparticles and hybridization chain reaction amplification［J］. Anal Chem，2013， 85（16）： 7689-7695.
44	ZHANG X， MENG H， LIU H， et al. Advances in laboratory detection methods and technology application of SARS-CoV-2［J］. J Med Virol，2022， 94（4）： 1357-1365.
45	SU W， LI J， JI C， et al. CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets［J］. Nano Res，2023， 16： 9940-9953.
46	LI H， XIE Y， CHEN F， et al. Amplification-free CRISPR/Cas detection technology： challenges， strategies， and perspectives［J］. Chem Soc Rev， 2023， 52： 361-382.
47	WU H， CHEN X， ZHANG M， et al. Versatile detection with CRISPR/Cas system from applications to challenges［J］. TrAC Trend Anal Chem，2021， 135： 116150.
48	YUAN C， TIAN T， SUN J， et al. Universal and naked-eye gene detection platform based on the clustered regularly interspaced short palindromic repeats/Cas12a/13a system［J］. Anal Chem，2020， 92（5）： 4029-4037.
49	TATON T A， MIRKIN C A， LETSINGER R L. Scanometric DNA array detection with nanoparticle probes［J］. Science，2000， 289（5485）： 1757-1760.
50	JI H， DONG H， YAN F， et al. Visual scanometric detection of DNA through silver enhancement regulated by gold-nanoparticle aggregation with a molecular beacon as the trigger［J］. Chemistry，2011， 17（40）： 11344-11349.
51	HAO N， LU J， ZHOU Z， et al. A pH-resolved colorimetric biosensor for simultaneous multiple target detection［J］. ACS Sens，2018， 3（10）： 2159-2165.
52	HO N R Y， LIM G S， SUNDAH N R， et al. Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes［J］. Nat Commun，2018， 9（1）： 3238.
53	ZHOU X， XIA S， LU Z， et al. Biomineralization-assisted ultrasensitive detection of DNA［J］. J Am Chem Soc，2010， 132（20）： 6932-6934.
54	PIAO J Y， PARK E H， CHOI K， et al. Direct visual detection of DNA based on the light scattering of silica nanoparticles on a human papillomavirus DNA chip［J］. Talanta，2009， 80（2）： 967-973.
55	SONG Y， WANG Y， QIN L. A multistage volumetric bar chart chip for visualized quantification of DNA［J］. J Am Chem Soc，2013， 135（45）： 16785-16788.
56	ZHU Z， GUAN Z， JIA S， et al. Au@Pt nanoparticle encapsulated target-responsive hydrogel with volumetric bar-chart chip readout for quantitative point-of-care testing［J］. Angew Chem Int Ed Engl，2014， 53（46）： 12503-12507.
57	WEI X， TIAN T， JIA S， et al. Target-responsive DNA hydrogel mediated “stop-flow” microfluidic paper-based analytic device for rapid， portable and visual detection of multiple targets［J］. Anal Chem，2015， 87（8）： 4275-4282.
58	WEI X， TIAN T， JIA S， et al. Microfluidic distance readout sweet hydrogel integrated paper-based analytical device （muDiSH-PAD） for visual quantitative point-of-care testing［J］. Anal Chem，2016， 88（4）： 2345-2352.
59	TIAN T， AN Y， WU Y， et al. Integrated distance-based origami paper analytical device for one-step visualized analysis［J］. ACS Appl Mater Interfaces，2017， 9（36）： 30480-30487.
60	NAJIAN A B N， SYAFIRAH E A R E N， ISMAIL N， et al. Development of multiplex loop mediated isothermal amplification （m-LAMP） label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira［J］. Anal Chim Acta，2016， 903： 142-148.
61	YAO M， LV X， DENG Y， et al. Specific and simultaneous detection of microRNA 21 and let-7a by rolling circle amplification combined with lateral flow strip［J］. Anal Chim Acta，2019， 1055： 115-125.
62	ZHENG X， WANG Y， BU S， et al. Point-of-care detection of 16S rRNA of Staphylococcus aureus based on multiple biotin-labeled DNA probes［J］. Mol Cell Probes，2019， 47（3）： 101427.
63	BROUGHTON J P， DENG X， YU G， et al. CRISPR-Cas12-based detection of SARS-CoV-2［J］. Nat Biotechnol，2020， 38（7）： 870-874.

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Application Research Progress of Visual Technique in Biomolecular Analysis

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