Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (2): 242-244.DOI: 10.11944/j.issn.1000-0518.2020.02.190215

• Communications • Previous Articles    

Comparison of Two Lactase Activity Determination Methods

HE Yunshana,LIU Yaweib,TAN Zhoujinb,HUI Huayinga*()   

  1. aCollege of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China
    bSchool of Medicine,Hunan University of Chinese Medicine,Changsha 410208,China
  • Received:2019-08-03 Accepted:2019-11-19 Published:2020-02-01 Online:2020-02-06
  • Contact: HUI Huaying
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.81703985)


Both o-nitrophenyl β-D-galactopyranoside(ONPG) method and disaccharase method are commonly used for the determination of lactase activity. This paper compared the linear range, detection limit, precision, accuracy and stability of these two methods. It is found that ONPG method is better than disaccharase method in linear range, detection limit, precision and stability, but this method has poor repeatability and needs further optimization.

Key words: o-nitrophenyl-β-D-galactopyranoside, disaccharase method, lactase activity, methodological comparison