应用化学 ›› 2020, Vol. 37 ›› Issue (3): 350-358.DOI: 10.11944/j.issn.1000-0518.2020.03.190256

• 研究论文 • 上一篇    下一篇

氮、铁共掺杂碳纳米粒子的制备及在过氧化氢和葡萄糖检测中的应用

孙莉娜a*(),李龑b,郭汉涛b,黄庭庭b,姚碧霞b,翁文bc   

  1. a漳州职业技术学院食品工程学院 福建 漳州 363000
    b闽南师范大学化学化工与环境学院 福建 漳州 363000
    c福建省现代分离分析科学与技术重点实验室 福建 漳州 363000
  • 收稿日期:2019-09-29 接受日期:2019-12-20 出版日期:2020-03-01 发布日期:2020-03-10
  • 通讯作者: 孙莉娜
  • 基金资助:
    福建省科技厅引导性项目(2016Y0065)漳州市科技重大专项(zz2018ZD10)和福建省中青年教师教育科研项目(JAT171093)资助

Preparation of Nitrogen and Iron Co-doped Carbon Nanoparticles and Their Applications in Detection of Hydrogen Peroxide and Glucose

SUN Li'naa*(),LI Yanb,GUO Hantaob,HUANG Tingtingb,YAO Bixiab,WENG Wenbc   

  1. aCollege of Food Engineering, Zhangzhou Institute of Technology,Zhangzhou,Fujian 363000,China
    bCollege of Chemistry,Chemical Engineering and Environment,Minnan Normal University,Zhangzhou,Fujian 363000,China
    cFujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology,Zhangzhou,Fujian 363000,China
  • Received:2019-09-29 Accepted:2019-12-20 Published:2020-03-01 Online:2020-03-10
  • Contact: SUN Li'na
  • Supported by:
    Supported by the Natural Science Foundation of Fujian Province of China(No.2016Y0065), the Major Science and Technology Projects of Zhangzhou City(No.zz2018ZD20), and the Education and Scientific Research Project for Young and Middle-aged Teachers of Fujian Province(No.JAT171093)

摘要:

L-酒石酸和一水柠檬酸为混合碳源,以乙二胺为氮源和聚合试剂,并添加六水三氯化铁,通过一锅溶剂热法合成了氮、铁共掺杂碳纳米粒子(N/Fe-CNPs),采用制备的N/Fe-CNPs模拟过氧化物酶催化过氧化氢(H2O2)氧化3,3',5,5'-四甲基 产生可溶性的蓝色产物,联合葡萄糖氧化酶建立了测定H2O2和葡萄糖含量的新方法。 结果显示:H2O2及葡萄糖的浓度与反应体系的吸光度呈良好的线性关系,H2O2的线性范围为0.2~20 μmol/L,葡萄糖的线性范围为0.1~1.0及1.0~80 μmol/L,最低检出限分别可达42.5和76.1 nmol/L。

关键词: 氮、铁共掺杂碳纳米粒子, 模拟过氧化物酶, 葡萄糖氧化酶, 过氧化氢检测, 葡萄糖检测

Abstract:

Nitrogen and iron co-doped carbon nanoparticles (N/Fe-CNPs) were synthesized by one-pot solvothermal method using L-tartaric acid and citric acid monohydrate as mixed carbon sources with the addition of ferric chloride hexahydrate, and ethylenediamine as the nitrogen source and polymerization reagent. The as-prepared N/Fe-CNPs could catalyze 3,3',5,5'-tetramethylbenzidine to produce the soluble blue product. Combined with glucose oxidase (GOx), a sensitive method for the detection of hydrogen peroxide and glucose was established. The concentration of hydrogen peroxide and glucose has a good linear relationship with the absorbance of the reaction system. The limit of detection was calculated to be 42.5 and 76.1 nmol/L for hydrogen peroxide and glucose, respectively.

Key words: nitrogen and iron co-doped carbon nanoparticles, peroxidase mimetics, glucose oxidase, detection of hydrogen peroxide, detection of glucose