氮、铁共掺杂碳纳米粒子的制备及在过氧化氢和葡萄糖检测中的应用

doi:10.11944/j.issn.1000-0518.2020.03.190256

摘要/Abstract

摘要：

以L-酒石酸和一水柠檬酸为混合碳源,以乙二胺为氮源和聚合试剂,并添加六水三氯化铁,通过一锅溶剂热法合成了氮、铁共掺杂碳纳米粒子(N/Fe-CNPs),采用制备的N/Fe-CNPs模拟过氧化物酶催化过氧化氢(H₂O₂)氧化3,3',5,5'-四甲基产生可溶性的蓝色产物,联合葡萄糖氧化酶建立了测定H₂O₂和葡萄糖含量的新方法。结果显示:H₂O₂及葡萄糖的浓度与反应体系的吸光度呈良好的线性关系,H₂O₂的线性范围为0.2~20 μmol/L,葡萄糖的线性范围为0.1~1.0及1.0~80 μmol/L,最低检出限分别可达42.5和76.1 nmol/L。

关键词: 氮、铁共掺杂碳纳米粒子, 模拟过氧化物酶, 葡萄糖氧化酶, 过氧化氢检测, 葡萄糖检测

Abstract:

Nitrogen and iron co-doped carbon nanoparticles (N/Fe-CNPs) were synthesized by one-pot solvothermal method using L-tartaric acid and citric acid monohydrate as mixed carbon sources with the addition of ferric chloride hexahydrate, and ethylenediamine as the nitrogen source and polymerization reagent. The as-prepared N/Fe-CNPs could catalyze 3,3',5,5'-tetramethylbenzidine to produce the soluble blue product. Combined with glucose oxidase (GOx), a sensitive method for the detection of hydrogen peroxide and glucose was established. The concentration of hydrogen peroxide and glucose has a good linear relationship with the absorbance of the reaction system. The limit of detection was calculated to be 42.5 and 76.1 nmol/L for hydrogen peroxide and glucose, respectively.

Key words: nitrogen and iron co-doped carbon nanoparticles, peroxidase mimetics, glucose oxidase, detection of hydrogen peroxide, detection of glucose

孙莉娜,李龑,郭汉涛,黄庭庭,姚碧霞,翁文. 氮、铁共掺杂碳纳米粒子的制备及在过氧化氢和葡萄糖检测中的应用[J]. 应用化学, 2020, 37(3): 350-358.

SUN Li'na,LI Yan,GUO Hantao,HUANG Tingting,YAO Bixia,WENG Wen. Preparation of Nitrogen and Iron Co-doped Carbon Nanoparticles and Their Applications in Detection of Hydrogen Peroxide and Glucose[J]. Chinese Journal of Applied Chemistry, 2020, 37(3): 350-358.

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