全固态酸度微电极组的设计与应用

doi:10.11944/j.issn.1000-0518.2019.06.180320

摘要/Abstract

摘要：

以木犀草素为pH特性敏感物,碳纤维为导电基质,固体石蜡为粘合剂制成了木犀草素碳纤维酸度微电极,将该电极与自制导电凝胶型全固态微型Ag/AgCl电极和铂丝微电极联用,构成全固态酸度微电极组。采用循环伏安法研究了该电极组对pH值为2.00~10.00的磷酸盐缓冲溶液有效酸度的响应,结果表明,循环伏安曲线上有一对可逆氧化还原峰,两峰的峰电势均随pH值呈线性变化,据此建立了方波伏安法测定溶液酸度的方法,氧化峰的峰电势(E_pa)与pH的线性回归方程为:E_pa(V)=-0.0567pH+0.603(r=0.999),体液中常见离子及蛋白质等不干扰测定。该电极组微型、无毒、抗干扰能力强,应用于微量体表唾液和汗液酸度的测定,取得了令人满意的结果,为微量体液的实时、在体、现场检测提供了技术基础。

关键词: 木犀草素修饰酸度微电极, 微型Ag/AgCl参比电极, 全固态, 方波伏安法

Abstract:

A micro-luteolin carbon fiber acidity microelectrode(Lu/CFME) was made with luteolin as a pH sensitive substance, carbon fiber as conductive matrix and solid paraffin as a binder. An all solid state acidity microelectrode set was combinated by Lu/CFME as working electrode, a self-made conductive gel type all-solid micro Ag/AgCl as reference electrode and a platinum wire microelectrode as auxiliary electrode. The response of the electrode set to the effective acidity was studied in the solution of phosphate buffer solution at pH 2.00~10.00 by cyclic voltammetry. The results show that there is a pair of reversible redox peaks on the cyclic voltammetry curve. Their peak potentials(E_pa) were all linear with pH. Based on this, a method for determining the acidity of solution was established by square wave voltammetry, and the linear regression equation for peak potential of oxidation peak and pH is E_pa(V)=-0.0567pH+0.603(r=0.999). The electrode set is micro, non-toxic and anti-interference. It is applied to acidity measurement of trace saliva and sweat on the skin surface, and satisfactory results have been obtained. Therefore, this result provides a technical basis for real-time, in-vivo detection of body fluids.

Key words: luteolin modified acidity microelectrode, micro Ag/AgCl reference electrode, all solid, square wave voltammetry

李玉莹, 代鑫, 赫春香. 全固态酸度微电极组的设计与应用[J]. 应用化学, 2019, 36(6): 711-716.

LI Yuying, DAI Xin, HE Chunxiang. Design and Application of All Solid State Acidity Microelectrode Set[J]. Chinese Journal of Applied Chemistry, 2019, 36(6): 711-716.

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