一步电沉积法构建辣根过氧化物酶传感器

doi:10.11944/j.issn.1000-0518.2015.07.140401

摘要/Abstract

摘要：

在恒电位沉积钴铝水滑石(CoAl-LDH)时将辣根过氧化物酶(HRP)和碳纳米管(CNTs)固定于基底电极表面,构建CoAl-LDH-HRP-CNTs修饰电极并用于过氧化氢的检测。利用SEM对电化学沉积的CoAl-LDH-HRP-CNTs的形貌进行了表征。采用电化学阻抗对所制备的电极进行了表征。用循环伏安法对电极的电化学行为进行了研究。探讨了pH值和测定电位对修饰电极的催化还原性能的影响。该传感器对过氧化氢的检测在2.5×10^-6~3.35×10^-4 mol/L范围内呈良好的线性关系,灵敏度为0.0049 A·L/mol。

关键词: 钴铝水滑石, 碳纳米管, 辣根过氧化物酶, 电沉积, 传感器

Abstract:

Horseradish peroxidase (HRP) and carbon nanotubes (CNTs) were co-immobilized on the base electrode surface during the process of potentiostatic electrodeposition of cobalt-aluminium layered double hydroxide (CoAl-LDH). The as-prepared CoAl-LDH-HRP-CNTs modified electrode was used for the determination of hydrogen peroxide. The morphology of CoAl-LDH-HRP-CNTs was characterized by SEM. The preparation process of the modified electrode was monitored by the electrochemical spectroscopy. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry. The effect of pH and work potential on the catalytic reduction performance of the modified electrode was investigated. The biosensor shows a good linear range of 2.5×10^-6~3.35×10^-4 mol/L for the determination of hydrogen peroxide with the sensitivity of 0.0049 A·L/mol.

Key words: cobalt-aluminium layered double hydroxide, carbon nanotubes, horseradish peroxidase, electrodeposition, biosensor

中图分类号:

杨绍明, 陈延胜, 李瑞琴, 龙启洋, 丁素游. 一步电沉积法构建辣根过氧化物酶传感器[J]. 应用化学, 2015, 32(7): 849-854.

YANG Shaoming, CHEN Yansheng, LI Ruiqin, LONG Qiyang, DING Suyou. One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition[J]. Chinese Journal of Applied Chemistry, 2015, 32(7): 849-854.

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