咪唑基取代β-环糊精聚合物固相萃取剂制备及分析应用

doi:10.3724/SP.J.1095.2014.30326

摘要/Abstract

摘要： 合成了单-6-脱氧-6-咪唑-β-环糊精聚合物（β-CDIMCP），建立了以β-CDIMCP为固相萃取剂分离分析碱性桃红T的新方法。结果表明，与β-环糊精聚合物(β-CDCP)相比，β-CDIMCP具有更好的吸附能力。室温下、pH=11.0时，β-CDIMCP能快速定量吸附碱性桃红T（吸附率95%）；以3.0 mL无水乙醇在8 min内可脱附碱性桃红T（脱附率90.0%）。在最佳条件下，β-CDIMCP对碱性桃红T富集倍数为10，检出限为4.9 μg/L，相对标准偏差3.4%(n=3,ρ=3.00 mg/L),线性范围为0.09~6.00 mg/L，相关系数R为0.9972。通过包合常数测定探讨了β-CDIMCP对碱性桃红T的吸附机理。采用该方法对实际样品中碱性桃红T含量测定，结果令人满意。

关键词: 碱性桃红T, 咪唑&, beta, -环糊精聚合物, 固相萃取, 紫外可见光谱法

Abstract: Mono-6-deoxy-6-imidazole-β-cyclodextrin polymer(β-CDIMCP) was synthesized and used as a new solid phase adsorbent to pre-concentrate/separate trace amount of safranine T in solutions. The results from UV-visible spectroscopy measurements show that β-CDIMCP had a better adsorption performance than β-CDCP. Safranine T was adsorbed by β-CDIMCP rapidly with an adsorption efficiency of 95% at room temperature and pH=11.0. When 3.0 mL of anhydrous ethanol was used as the eluant, the elution efficiency reached 90% in 8 minutes. Under the optimum condition, the preconcentration factor of the proposed method was 10. The detection limit(DL), RSD, linear range, and correlation coefficient was found to be 4.9 μg/L, 3.4%(n=3, ρ=3.00 mg/L), 0.09~6.0 mg/L and 0.9972, respectively. This proposed method was successfully applied to the determination of safranine T in real samples. The molecular interaction between β-CDIMCP and safranine T was studied through the inclusion constant study.

Key words: safranine T, imidazole-&, beta, -cyclodextrin polymer, solid-phase extraction, ultra-violet spectroscopy

中图分类号:

O657.7

周楠, 朱霞石. 咪唑基取代β-环糊精聚合物固相萃取剂制备及分析应用[J]. 应用化学, DOI: 10.3724/SP.J.1095.2014.30326.

ZHOU Nan, ZHU Xiashi*. Preparation of Imidazolyl Substituted β-Cyclodextrin Polymers as a Solid-phase Extractant and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30326.

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