应用化学

• 研究论文 • 上一篇    下一篇

快速生化需氧量微生物传感器的应用

赵利民1,郏建波2,刘长宇2*   

  1. (1.长春市环境监测中心站 长春 130022;2.中国科学院长春应用化学研究所 长春 130022)
  • 收稿日期:2011-11-09 修回日期:2012-01-16 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 刘长宇,助理研究员; Tel:0431-85262748; Fax:0431-85689711; E-mail:cyliu@ciac.jl.cn; 研究方向:污水在线监测方法

Application of Rapid Biochemical Oxygen Demand Biosensor

ZHAO Limin1, JIA Jianbo2, LIU Changyu2*   

  1. (1.Changchun Environmental Monitoring Center,Changchun 130022,China;
    2.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)
  • Received:2011-11-09 Revised:2012-01-16 Published:2012-07-10 Online:2012-07-10

摘要: 采用混合菌BODseed制备微生物传感器快速监测生化需氧量(BOD),研究了矫正溶液选择、样品自降解及恒温装置对测量准确度的影响。 结果表明,采用葡萄糖谷氨酸矫正溶液测得污水处理厂污水结果较BOD5方法偏低50%以上,而以污水本身制作矫正曲线取得了与BOD5一致的测试结果;样品在保存6 h后有机物自降解会使测量结果偏低40%;快速BOD在线监测时不仅需要对微生物传感器恒温,同时也需要对样品及缓冲溶液恒温,这有利于样品快速接近缓冲溶液温度,减少空气饱和时间,从而提高监测效率,提升测试准确度。

关键词: 生化需氧量, 微生物传感器, 恒温装置, 在线, 矫正溶液

Abstract: Multiple-species BODseed was used for fabricating biochemical oxygen demand(BOD) biosensors for rapid online BOD determination. Some factors to accuracy were studied such as calibration solution selection, sample self-biodegradation, and thermostatic device. Good results were obtained when wastewater was used as calibration solution instead of glucose and glutimic acid(GGA). The self-biodegradation process made the rapid measurements inaccurate. Besides, thermostatic device used only for biosensors is defective, and it should be applied for maintaining a constant temperature of the samples and buffer, promoting the efficiency and accuracy of online monitoring.

Key words: Biochemical oxygen demand, Biosensor, Thermostatic device, Online, Calibration solution

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