中华医学超声杂志

应用化学 ›› 1998, Vol. 0 ›› Issue (3): 63-66.

• 研究论文 • 上一篇    下一篇

EPR法研究自由基在移植免疫中的作用

袁毅1, 赵世洪1, 张瑞雪1, 袁硕2   

  1. 1. 白求恩医科大学眼科 长春130021;
    2. 黑龙江乳品检测中心 哈尔滨
  • 收稿日期:1998-01-12 修回日期:1998-05-15 出版日期:1998-06-10 发布日期:1998-06-10

Study on the Action of Free Radical in Graft Immunization by Electron Paramagnetic Resonance Method

Yuan Yi1, Zhao Shihong1, Zhang Ruixue1, Yuan Shou2   

  1. 1. Ophthalmic Department, Norman Bethune University of Medical Sciences, Changchun 130021;
    2. Heilongjiang Dairy Industry Development Centre, Harbin
  • Received:1998-01-12 Revised:1998-05-15 Published:1998-06-10 Online:1998-06-10

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摘要： 用电子顺磁共振(EPR)法,化学发光法及荧光光谱法研究了100例正常人、42例角膜移值患者泪液中的自由基、超氧化物岐化酶(SOD)和水溶性脂质过氧化物(WSLP)等指标。结果表明正常人泪液中自由基的自旋浓度是(3.6±0.58)×1012spins/mL,SOD浓度为(3.84±1.45)μg/L、水溶性脂质过氧化物相对荧光强度为9.598～16.225U/mL.角膜移植后三项指标变化出现两个规律的峰,这种规律性变化在三项指标间存在显着的相关性。其中5例患者的特殊变化证明移植排斥反应与术后这些变化的进一步持续发展有关

关键词: 电子顺磁共振, 自由基, 超氧化物岐化酶, 水溶性脂质过氧化物, 角膜移植

Abstract: By using the methods of Electron Paramagnetic Resonance(EPR)、chemilumineseence and fluoresence spectrophotometry, the contents of free radicals、superoxide dismutases(SOD) and water soluble lipid peroxides in tears in the normal eyes(100 cases) and keratoplasty eyes(42 cases) have been investigated. The results indicated that the spin density of free radicals was (3.6±0.58)×1012 spins/mL, SOD was (3.84±1.45) μg/L, water soluble lipid peroxide was (12.912±1.691) U/mL in the normal tears. Two peaks were found in every target after 24 hours and 21 day of the keratoplasty operation. Both peaks regularily. An obvious interrelation was there in every pair of the three targets. However, the special changes of 5 cases of 42 cases proved free radicals and lipid peroxides increased continuously and the rejection was inereased. These findings suggest that there is free radical reaction mechanism in the rejection and that after keratoplasty supervising and determining free radical、SOD and water soluble lipid peroxide are important for preventing the rejection.

Key words: electron paramagnetic resonance, free radical, superoxide dismutase, water soluble lipid peroxide, keratoplasty

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