应用化学 ›› 1996, Vol. 0 ›› Issue (6): 28-30.

• 研究论文 • 上一篇    下一篇

环糊精葡萄糖基转移酶的固定化及其应用──Ⅲ.固定化环糊精葡萄糖基转移酶制备环糊精的研究

朱伯儒, 史作清, 何炳林   

  1. 南开大学高分子化学研究所, 吸附分离功能高分子材料国家重点实验室 天津 300071
  • 收稿日期:1996-02-25 修回日期:1996-08-07 出版日期:1996-12-10 发布日期:1996-12-10

Immobilization of Cyclodextrin Glycosyltransferase and Its Usage──Ⅲ.Study on Preparation of Cyclodextrin Using Immobilized Cyclodextrin Glycosyltransferase

Zhu Boru, Shi Zuoqing, He Binglin   

  1. Institute of Polymer Chemistry, Nankai University, The State Key Laboratory of, Functional Polymer Materials for Ad, Tianjin 300071
  • Received:1996-02-25 Revised:1996-08-07 Published:1996-12-10 Online:1996-12-10

摘要: 用固定化环糊精葡萄糖基转移酶催化淀粉环化反应,考察了反应时间、乙醇浓度和淀粉液化程度对环糊精产率的影响。环糊精产率最高达54%,固定化酶经多次重复使用或长时间连续操作,酶活力降低较少,产品经纸色谱分离后用碘显色,发现产品主要为β-环糊精。

关键词: 固定化, 环糊精葡萄糖基转移酶, 环糊精

Abstract: The immobilized cyclodextrin glycosyltransferase(CGTase) was used for preparation of cyclodextrin(CD).The influence of reaction time,concentration of alcohol and liquification degree of starch on yield of CD was discussed.The highest yield of CD could reach 54% and the main product was β-CD.The activity of immobilized CGTase lost very little after repeated use.

Key words: immobilization, cyclodextrin glycosyltransferase, cyclodextrin