中华医学超声杂志

应用化学 ›› 1996, Vol. 0 ›› Issue (6): 24-27.

• 研究论文 • 上一篇    下一篇

α－葡萄糖基甜菊糖的酶促合成反应(Ⅱ)

史作清1, 朱伯儒1, 施荣富1, 李小婷1, 何炳林1, 贾志芬2, 乔金胜2   

  1. 1. 南开大学高分子化学研究所, 吸附分离功能高分子材料国家重点实验室 天津 300071;
    2. 南开大学合成科技发展公司 天津
  • 收稿日期:1996-03-30 修回日期:1996-08-07 出版日期:1996-12-10 发布日期:1996-12-10

Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)

Shi Zuoqing1, Zhu Boru1, Shi Rongfu1, Li Xiaoting1, He Binglin1, Jia Zhifen2, Qiao Jinsheng2   

  1. 1. Institute of Polymer Chemistry, Nankai University, The State Key Laboratory of Functional Polymer Materials for Ad, Tianjin 300071;
    2. Hecheng High Science and Technology Developing Cmnpany, Nankai University, Tianjin
  • Received:1996-03-30 Revised:1996-08-07 Published:1996-12-10 Online:1996-12-10

PDF

547

摘要： 研究了以球形纤维素弱阴离子交换剂作载体,固定化葡萄糖基转移酶GT-1转化甜菊糖为α-葡萄糖基甜菊糖反应的各种影响因素。结果表明,甜菊糖的酶促转化到达一定程度后,继续增大酶用量及淀粉用量,对转化结果没有太多改善,固定化酶重复使用8次,活力下降较少,说明固定化效果良好。

关键词: 甜菊糖, 固定化, 葡萄糖基转移酶, 酶促转化

Abstract: Anion cellulosic beads were used as support for immobilization of glycosyltransferase GT-1.The immobilized enzyme was used to catalyze the synthesis of α-glycosylstevia sugar.Various influencing factors were discussed.The result showed that when the reaction reached to a certain extent,transglycosylated amount changed very little with increase of amounts of enzyme and starch.The activity of immobilized GT-1 lost very little after repeated uses for 8 times.

Key words: stevia sugar, immobilization, glycosyltransferase, enzyme-catalyzed synthesis

网站版权 © 应用化学     本网站备案：吉ICP备12000082号