四环素-Ag复合材料的协同抑菌性能

doi:10.19894/j.issn.1000-0518.210006

摘要/Abstract

摘要： 以三氟醋酸银(CF₃COOAg)为原料,在硫氢化钠(NaHS)、氯化氢(HCl)和聚乙烯吡咯烷酮(PVP)的一缩二乙二醇溶液中制备纳米银(Ag NPs)颗粒。利用透射电子显微镜(TEM),X射线衍射仪(XRD)、紫外-可见漫反射光谱(UV-Vis DRS)和X射线光电子能谱(XPS)对其进行表征。以大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)为模式菌,用滤纸片扩散和菌落计数法研究纳米Ag和市售四环素协同抑菌性能。结果表明,四环素中协同50 μg/mL Ag NPs具备超强的抑菌性能,对模式菌-革兰氏阴性菌大肠杆菌(E.coli)的抑菌效率比单独四环素抑菌效率提高了31.3%,对革兰氏阳性菌金黄色葡萄球菌(S.aureus)的抑菌性能增强24.5%,且对细胞壁中含有更多蛋白质的E.coli更为敏感,其优越的抑菌活性可应用于医疗领域。

关键词: 协同, 抑菌, 银, 抑菌机理, 配位

Abstract: Ag nanoparticles were successfully prepared from CF₃COOAg in the presence of NaSH, HCl and polyvinylpyrrolidone in diethylene glycol solvent. The nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible diffuse reflectance spectra (UV-Vis DRS), and X-ray photoelectron spectrometry (XPS). The combined antibacterial activities of Ag nanoparticles (NPs) and commercially available tetracycline against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) by the disc diffusion assay and the colony count were evaluated. Strong synergistic antimicrobial effect of Ag NPs and tetracycline has been observed. The antimicrobial activity of tetracycline is improved by 31.3% and 24.5% to E.coli and S.aureus, respectively, in the presence of 50 μg/mL of Ag NPs, and is more sensitive to E.coli. The reason could be mainly ascribed to the presence of more proteins in the cell wall of E.coli than that of S.aureus. The results of this study demonstrate potential therapeutic applications of tetracycline in combination with Ag NPs.

Key words: Synergy, Bacteriostasis, Silver, Bacteriostasis mechanism, Coordination

中图分类号:

O614.1

郭少波, 梁艳莉, 刘智峰, 张强, 马剑琪. 四环素-Ag复合材料的协同抑菌性能[J]. 应用化学, 2021, 38(11): 1462-1468.

GUO Shao-Bo, LIANG Yan-Li, LIU Zhi-Feng, ZHANG Qiang, MA Jian-Qi. Synergistic Antibacterial Activity of Tetracycline Combined with Silver Nanoparticles[J]. Chinese Journal of Applied Chemistry, 2021, 38(11): 1462-1468.

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