毛细管电色谱-电喷雾-飞行时间/质谱联用分离分析混合氨基酸对映体

doi:10.11944/j.issn.1000-0518.2019.07.190021

摘要/Abstract

摘要：

建立了一种简单高效的毛细管电色谱-电喷雾-飞行时间/质谱(CEC-ESI-TOF/MS)联用技术分离混合氨基酸对映体的方法。以D,L-精氨酸、D,L-缬氨酸和D,L-谷氨酸为研究对象,通过优化CEC分离条件和MS检测条件,3种混合氨基酸对映体的6个组分在15 min内实现了分离,分离度分别为3.03、1.59和1.37。该方法为混合对映体的分离分析提供了参考方法和基础数据。

关键词: 毛细管电色谱-电喷雾-飞行时间/质谱, 对映体拆分, 混合氨基酸, 整体柱

Abstract:

A simple and efficient capillary electrochromatography-electrospray- time of flight/mass spectrometry(CEC-ESI-TOF/MS) method for the separation and analysis of mixed amino acid enantiomers was established. D,L-arginine, D,L-valine, and D,L-glutamate acid were taken as the research objects. By optimizing the CEC separation conditions and MS detection conditions, the six components of the three mixed amino acid enantiomers were separated within 15 min, and the resolutions were 3.03, 1.59 and 1.37, respectively. It provides a reference method for the separation and analysis of mixed enantiomers.

Key words: capillary electrochromatography-electrospray-time of flight/mass spectrometry, enantiomer separation, mixed amino acid, monolithic column

李英杰,林肖同,高立娣,秦世丽,唐艺旻,靳志向,张帅. 毛细管电色谱-电喷雾-飞行时间/质谱联用分离分析混合氨基酸对映体[J]. 应用化学, 2019, 36(7): 823-831.

LI Yingjie,LIN Xiaotong,GAO Lidi,QIN Shili,TANG Yimin,JIN Zhixiang,ZHANG Shuai. Separation and Analysis of Mixed Amino Acid Enantiomers by Capillary Electrochromatography-Electrospray- Time of Flight/Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, 2019, 36(7): 823-831.

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