线性麦芽糊精聚合物功能化Fe3O4纳米复合物药物载体的合成和应用

doi:10.11944/j.issn.1000-0518.2019.02.180204

摘要/Abstract

摘要：

采用共沉淀法制备得到了线性麦芽糊精聚合物功能化的Fe₃O₄磁性纳米粒子(LM-SP-MNPs),通过傅里叶变换红外光谱、透射电子显微镜、热重分析等技术对其结构、形貌进行了表征。其粒径大小为(12±2) nm。选取抗癌药物盐酸阿霉素(DOX)作为模型药物,运用荧光光谱法研究了LM-SP-MNPs的载药性能和释放行为,探讨了pH值对LM-SP-MNPs药物释放性能的影响。最适pH条件下,LM-SP-MNPs对盐酸阿霉素的最大吸附量约为357.1 mg/g,吸附等温线符合Freundlich等温吸附模型。 LM-SP-MNPs与盐酸阿霉素的复合物(DOX@LM-SP-MNPs),在37 ℃的条件下药物在酸性条件下的释放效率大于中性条件。 pH=5.3时,盐酸阿霉素在7 h内的累积释放率为26.9%。此外,细胞毒性试验表明,LM-SP-MNPs具有良好的生物相容性,而DOX@LM-SP-MNPs和肝癌细胞共培养后可以明显杀死HepG2肝癌细胞。

关键词: 线性麦芽糊精聚合物, Fe₃O₄磁性纳米粒子, 盐酸阿霉素, 载药

Abstract:

Linear maltodextrin polymer modified magnetic nanoparticles(LM-SP-MNPs) were prepared by chemical co-precipitation. The structure and morphology of LM-SP-MNPs were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy and thermogravimetric analysis. The loading and release behaviors of LM-SP-MNPs as drug carrier were evaluated by fluorescence spectroscopy using doxorubicin(DOX) as a model drug. The pH effects on LM-SP-MNPs for drug releasing were investigated. Under the optimal pH conditions, the maximum loading of DOX into LM-SP-MNPs is 357.1 mg/g, and the loading behavior follows Freundlich adsorption balance equations with multilayer adsorption. Furthermore, the release ability of DOX@LM-SP-MNPs is more efficient under acid solution than that under natural solution at 37 ℃ in vitro. Accumulated release efficiency of DOX in 7 h is 26.9% at pH=5.3. In addition, the MTT assays show that LM-SP-MNPs have an excellent biocompatibility, and the DOX@LM-SP-MNPs decrease the relative cellular viability of HepG2 cells evidently.

Key words: Linear maltodextrin polymer, Fe₃O₄ magnetic nanoparticle, doxorubicin, drug carrier

弓韬, 黄昱, 郭国英, 苏丹, 梁文婷, 董川. 线性麦芽糊精聚合物功能化Fe₃O₄纳米复合物药物载体的合成和应用[J]. 应用化学, 2019, 36(2): 161-169.

GONG Tao, HUANG Yu, GUO Guoying, SU Dan, LIANG Wenting, DONG Chuan. Linear Maltodextrin Polymer Functionalized Fe₃O₄ Magnetic Nanoparticles as Drug Carriers[J]. Chinese Journal of Applied Chemistry, 2019, 36(2): 161-169.

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线性麦芽糊精聚合物功能化Fe₃O₄纳米复合物药物载体的合成和应用

Linear Maltodextrin Polymer Functionalized Fe₃O₄ Magnetic Nanoparticles as Drug Carriers

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