血红蛋白/光敏剂复合药物体系用于光动力治疗

doi:10.11944/j.issn.1000-0518.2018.12.180102

摘要/Abstract

摘要：

通过等电点法实现了血红蛋白(Hb)与光敏剂药物七甲川花菁类小分子:11-氯-1,1'-二正丙基-3,3,3',3'-四甲基-10,12-三亚甲基吲哚三碳花青碘盐(IR780)的共担载,并研究了Hb供氧治疗与光动力治疗的联合治疗效果。通过透射电子显微镜和动态光散射研究了Hb/IR780复合药物载体的形貌与稳定性,证明了药物载体在生理条件下能够稳定存在。通过对药物在体外溶液和细胞水平的活性氧(ROS)检测,验证了Hb供氧能够有效地促进光敏剂ROS的产生,并且细胞毒性实验也证实了Hb/IR780复合药物载体拥有比单组份IR780药物更明显的肿瘤细胞杀伤效果。

关键词: 血红蛋白, 光动力治疗, 氧气治疗

Abstract:

In this paper, the loading of both hemoglobin and photosensitizer drug heptamethine cyanine fluorescent small molecule:11-chloro-1,1'-di-n-propyl-3,3,3',3'-tetramethyl-10,12-trimethylene quinone three-carbon cyanine iodide salt(IR780) was achieved by the isoelectric point method. The effect of the combined treatment of hemoglobin oxygen therapy and photodynamic therapy was studied. The morphology and stability of the hemoglobin/IR780 complex drug carrier were studied by transmission electron microscopy and dynamic light scattering, proving that the drug carrier maintains good stability under physiological conditions. The reactive oxygen species(ROS) detection of the drug in the solution and cell confirmed that the oxygen supply of hemoglobin could promote the production of ROS effectively. Meanwhile, it was also confirmed that the hemoglobin/IR780 complex drug carrier was more efficient to kill tumor cells than the single component IR780 drug in cell cytotoxicity experiments.

Key words: hemoglobin, photodynamic therapy, oxygen therapy

王玉鹏, 周东方, 程延祥, 黄宇彬. 血红蛋白/光敏剂复合药物体系用于光动力治疗[J]. 应用化学, 2018, 35(12): 1442-1448.

WANG Yupeng, ZHOU Dongfang, CHENG Yanxiang, HUANG Yubin. Hemoglobin/Photosensitizer Compound Drug System for Photodynamic Therapy[J]. Chinese Journal of Applied Chemistry, 2018, 35(12): 1442-1448.

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