甲苯咪唑与曙红Y相互作用的共振瑞利散射、倍频散射和荧光光谱及其分析应用

doi:10.11944/j.issn.1000-0518.2015.04.140281

摘要/Abstract

摘要：

在pH值3.4~3.9的Britton-Robinson(BR)缓冲介质中,甲苯咪唑(MBZ)与曙红Y(EY)反应形成1:1的离子缔合物,体系反应不仅导致荧光光谱的猝灭,还使共振瑞利散射(RRS)和倍频散射(FDS)显著增强,最大的RRS峰位于326 nm处。荧光猝灭法、RRS法、FDS法的检出限分别为32.31、7.24和11.65 μg/L,其中RRS法的灵敏度最高。实验讨论了反应的最佳条件以及共存物质的影响。该方法用于甲苯咪唑片剂以及尿样中MBZ的测定,结果令人满意。

关键词: 共振瑞利散射, 倍频散射, 荧光猝灭, 甲苯咪唑, 曙红Y

Abstract:

In pH 3.4~3.9 Britton-Robinson(BR) buffer solution, mebendazole(MBZ) reacts with eosin Y(EY) to form a 1:1 ion-association complex, which not only results in the quenching of fluorescence, but also results in the great enhancement of resonance Rayleigh scattering(RRS) and frequency doubling scattering(FDS). Furthermore, a new RRS spectrum appears, and the maximum RRS wavelength is located at 326 nm. The detection limit for MBZ is 32.31 μg/L using the fluorophotometry, 7.24 μg/L by the RRS method and 11.65 μg/L by the FDS method. Among them, the RRS method has the highest sensitivity. The experimental conditions were optimized and effects of coexisting substances were evaluated. These methods have been applied to the determination of MBZ in tablets and human urine samples. The composition and structure of the ion-association complex and the reaction mechanism are discussed.

Key words: resonance rayleigh scattering, frequency doubling scattering, fluorescence quenching, mebendazole, Eosin Y

中图分类号:

O657

李勤, 田丰玲, 周尚, 傅凌莉, 刘蕖, 杨季冬. 甲苯咪唑与曙红Y相互作用的共振瑞利散射、倍频散射和荧光光谱及其分析应用[J]. 应用化学, 2015, 32(4): 481-488.

LI Qina, , TIAN Fenglingb, ZHOU Shangc, FU Linglia, LIU Qua, YANG Jidongd. Resonance Rayleigh Scattering, Frequency Doubling Scattering and Fluorescence Spectra of the Eosin Y-Mebendazole System and Their Analytical Applications[J]. Chinese Journal of Applied Chemistry, 2015, 32(4): 481-488.

参考文献

[1] GUO Dongfang,LIU Yongtao,AI Xiaohui, et al . Pharmacokinetics of Mebendazole in Johnny Carp(Carassius auratus gibelio)[J]. J Fishery Sci China ,2009,16(3):434-441(in Chinese). 郭东方,刘永涛,艾晓辉,等. 甲苯咪唑在银鲫体内的药代动力学研究[J]. 中国水产科学,2009,16(3):434-441.
[2] LIU Rongxing,QI Lijun,LI Xiangyu. Therapeutic Efficacy of Mebendazole Composite Cream Against Pig Intestinal Nematodes[J]. Academic J Second Military Medical Univ ,2003,24(12):1279-1280(in Chinese). 刘荣兴,戚丽君,李翔宇. 复方甲苯咪唑乳膏对猪肠道线虫的疗效[J]. 第二军医大学学报,2003,24(12):1279-1280.
[3] Pantelis K,Nikos P,Pascal D. Treatment of Microcotyle sp.(Monogenea) on the Gills of Cage-cultured Red Porgy, Pagrus Pagrus Following Baths with Formalin and Mebendazole[J]. Aquaculture ,2006,251(2/4):167-171.
[4] EEC Council Regulation No.2377 /90. Laying Down a Community Procedure for the Establishment of Maximum Residuelimits of Veterinary Medicinal Products in Foodstuffs of Animal Origin[J]. Off J Eur Commun ,No.L 244/1[S].1990.
[5] YIN Rong. HPLC Determination of Compound Mebendazole Tablets[J]. Nat Med Front China ,2010,5(10):68-69(in Chinese). 尹嵘. 高效液相色谱法测定复方甲苯咪唑片剂的含量[J]. 药物与临床,2010,5(10):68-69.
[6] LIU Yongtao,AI Xiaohui,YANG Hong. Simultaneous Determination of Mebendazole and Its Metabolites Residues in Fishery Products by High Performance Liquid Chromatography Tandem Mass Spectrometry with Isotope Dilution[J]. J Instrum Anal ,2011,30(6):640-645(in Chinese). 刘永涛,艾晓辉,杨红. 同位素稀释高效液相色谱-串联质谱法测定水产品中甲苯咪唑及其代谢物的残留量[J]. 分析测试学报,2011,30(6):640-645.
[7] LIN Jing,ZHANG Jinhu,LIN Yonghui, et al . Determination of Florfenicol and Mebendazole Residues in Aquatic Product by High Performance Liquid Chromatography[J]. Agrochemicals ,2012,51(5):364-368(in Chinese). 林荆,张金虎,林永辉,等. 高效液相色谱法测定水产品中氟苯尼考和甲苯咪唑的残留量[J]. 农药,2012,51(5):364-368.
[8] CHENG Xuemei,WANG Changhong,DIAN Jia. Determination of Mebendazole Formulations and Drug Content[J]. Endemic Dis Bull ,2001,16(2):89-92(in Chinese). 程雪梅,王长虹,殿甲. 甲苯咪唑制剂及体内药物含量测定[J]. 地方病通报,2001,16(2):89-92.
[9] YU Huijuan,FENG Bing,JIANG Chaojun, et al . Determination of Residues of Mebendazole and Its Metabolites in Fish Pshrimp by Solid Phase Extraction-High Performance Liquid Chromatography[J]. Chinese J Anal Lab ,2010,29(4):33-36(in Chinese). 于慧娟,冯兵,姜朝军,等. 固相萃取-高效液相色谱法测定鱼P虾生物体中甲苯咪唑及代谢物的残留[J]. 分析试验室,2010,29(4):33-36.
[10] JIANG Chaojun,YU Huijuan,CAI Youqiong, et al . Determination of Mebendazole and Its Metabolites in Fishery by HPLC-UV[J]. Food Ferment Ind ,2009,35(12):130-133(in Chinese). 姜朝军,于慧娟,蔡友琼,等. 高效液相色谱法测定水产品中甲苯咪唑及其代谢物残留[J]. 食品与发酵工业,2009,35(12):130-133.
[11] GUO Dongfang,LIU Yongtao,AI Xiaohui, et al . Simultaneous Determination of Mebendazole and Its Metabolites in Johnny Carp(Carassius Auratus Gibelio) Muscle by HPLC /UV Method[J]. J Instrum Anal ,2009,28(4):493-496(in Chinese). 郭东方,刘永涛,艾晓辉,等. HPLC/UV法对银鲫肌肉组织中甲苯咪唑及其代谢物残留的同时分析[J]. 分析测试学报,2009,28(4):493-496.
[12] Hajee C A,Haasma N. Liquid Chromatographic Determination of Mebendazole and Its Metabolites, Aminomebendazole and Hydroxymebendazole, in Eel Muscle Tissue[J]. J AOAC Int ,1996,79(3):645-651.
[13] WANG Erkang. The Progress of Analytical Chemistry [M]. Beijing:Science Press,2002:280-288(in Chinese). 汪尔康. 分析化学新进展[M]. 北京:科学出版社,2002:280-288.
[14] Huang C Z,Li Y F. Resonance Light Scattering Technique Used for Biochemical and Pharmaceutical Analysis[J]. Anal Chim Acta ,2003,500:105-117.
[15] GAO Xiangyang,XU Yali,ZHANG Na, et al . Determination of Neomycin in Milk with Acidic Fuchsin-B-R System by Resonance Light Scattering Method[J]. J Food Sci ,2013,34(6):219-222(in Chinese). 高向阳,徐雅莉,张娜,等. 酸性品红-B-R体系共振光散射法测定牛奶中的新霉素[J]. 食品科学,2013,34(6):219-222.
[16] HAN Quan,TIAN Li,HUO Yanyan, et al . Determination of Sodium Alginate with Janus Green as a Probe by Resonance Rayleigh Scattering Spectrometry[J]. J Anal Sci ,2013,29(4):473-477(in Chinese). 韩权,田丽,霍燕燕,等. 健那绿作光谱探针共振瑞利散射法测定藻酸钠[J]. 分析科学学报,2013,29(4):473-477.
[17] ZHANG Min,YU Yongli,WANG Limei, et al . Determination of Human Serum Albumin Based on Fluorescence Enhancement of Tb 3+ -Sarafloxacin Complexes[J]. Chinese J Anal Lab ,2013,32(2):22-25(in Chinese). 张敏,于永丽,王丽美,等. 基于铽-沙拉沙星配合物的荧光增强测定人血清白蛋白[J]. 分析试验室,2013,32(2):22-25.
[18] SONG Zhongrong,ZOU Rong,HE Jiahong, et al . Resonance Light Scattering Spectral Method for Determination of Trace Errum(Ⅲ) with Acridine Red[J]. J Appl Chem ,2012,29(5):597-603(in Chinese). 宋仲容,邹容,何家洪,等. 吖啶红共振散射光谱法测定痕量铁(Ⅲ)[J]. 应用化学,2012,29(5):597-603.