应用化学 ›› 2010, Vol. 27 ›› Issue (08): 903-906.DOI: 10.3724/SP.J.1095.2010.90646

• 研究论文 • 上一篇    下一篇

三羟甲基氨基甲烷缩2-吡啶甲醛席夫碱铜配合物的合成、表征及与DNA相互作用

杨新斌1*,张金生2,陈朝晖1,曾仁权1   

  1. (1.西南大学荣昌校区基础部 重庆 402460;2.贵州师范大学化学与材料科学学院 贵阳)
  • 收稿日期:2009-10-06 修回日期:2009-12-02 出版日期:2010-08-10 发布日期:2010-08-10
  • 通讯作者: 杨新斌,男,博士,副教授; E-mail:yangxbqq@126.com; 研究方向:有机合成及化学生物学

Synthesis and Characterization of A New Schiff Base Copper(Ⅱ) Complex from Tris(hydroxymethyl)aminomethane and 2-Pyridinecarboxaldehyde, and Its Interaction with DNA

YANG Xin-Bin1*, ZHANG Jin-Sheng2, CHEN Zhao-Hui1, ZENG Ren-Quan1   

  1. (1.Department of Basic Science,Rongchang Campus,Southwest University,Chongqing 402460;
    2.School of Chemistry and Material Science,Guizhou Normal University,Guiyang)
  • Received:2009-10-06 Revised:2009-12-02 Published:2010-08-10 Online:2010-08-10

摘要:

合成了三羟甲基氨基甲烷缩2-吡啶甲醛席夫碱铜配合物[C10H14CuN2O3]Cl2,通过元素分析、摩尔电导、红外光谱和高分辨质谱等测试技术对化合物进行了结构表征。利用电子吸收光谱、热变性和粘度实验研究了配合物与CT-DNA的相互作用,实验结果表明,该配合物以嵌插模式与DNA相互作用。

关键词: 铜配合物, 小牛胸腺DNA, 嵌插

Abstract:

A schiff base copper complex prepared through the condensation between tris(hydroxymethyl)aminomethane and 2-pyridinecarboxaldehyde was characterized by elemental analysis, molar conductance, IR and HRMS. The interaction of the complex with culf thymus(CT-DNA) was investigated by UV spectra, viscosity measurements and DNA thermal denaturation experiments. The experimental results show that the mode of the complex bound to DNA is intercalation.

Key words: Copper complex, CT-DNA, inercalation

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