中华医学超声杂志

应用化学 ›› 1998, Vol. 0 ›› Issue (1): 56-58.

• 研究论文 • 上一篇    下一篇

用毛细管区带电泳法研究水稻种子胚乳贮藏蛋白

张滨1, 刘莉丽1, 林炳承1, 张亚兵2, 盛海平2   

  1. 1. 中国科学院大连化学物理研究所 大连 116023;
    2. 安徽省种子质量监督检验站 合肥
  • 收稿日期:1997-04-07 修回日期:1997-09-17 出版日期:1998-02-10 发布日期:1998-02-10
  • 基金资助:
    国家自然科学基金

Study of Rice Seed Endosperm Storage Proteins by Capillary Zone Electrophoresis

Zhang Bin1, Liu Lili1, Lin Bingcheng1, Zhang Yabing2, Sheng Haiping2   

  1. 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023;
    2. Anhui Provincial Seed Inspection Agency, Hefei
  • Received:1997-04-07 Revised:1997-09-17 Published:1998-02-10 Online:1998-02-10

PDF

403

被引次数

2

摘要： 水稻品种鉴别目前常采用种子形态分析、蛋白和同工酶的聚丙烯酞胺凝胶电泳分析等方法,其缺点是试剂有毒,分析时间长,且很难区别亲缘关系接近的同母异父杂交水稻种子.毛细管电泳是一种高效分离技术[1,2],具有快速、微量、自动化等优点.本文采用毛细管区带电泳(CZE)对国产13种水稻种子胚乳贮藏蛋白进行初步研究,探讨了作为品种鉴别模式的实际可操作性,以期能为品种鉴别提供新的有效手段.

关键词: 水稻种子, 胚乳贮藏蛋白, 醇溶蛋白, 毛细管区带电泳, 鉴别

Abstract: Prolamins from thirteen kinds of homemade rice seed have been preliminary stu-dled by capillary zone electrophoresis(CZE).The results showed that CZE can be used as asupplement method for comparing and differentiating the rice cultivars, especially some hy-brids, which are difficult to differentiate by polyacrylamide gel electrophoresis.

Key words: rice seed, endosperm storage protein, prolamine, capillary zone electrophoresis, characterization

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